|
571. Protein analog - (reply: 1)
572. membrane protein extraction buffer - (reply: 1)
573. What about the Baculovirus expression system? - (reply: 2)
574. Ni-NTA beads and dialysis tubing - Purification using nickel beads (reply: 4)
575. Concentrating Protein with centrifuge concentrator but 50% glycerol!!? - (reply: 2)
576. protein is degraded after purification - (reply: 2)
577. protein expression - Preparation of starter cultures (reply: 15)
578. Problem with Protein elution using GST sepharose 4B - (reply: 1)
579. how to isolate insoluble protein in bacterial induction? - (reply: 4)
580. Plasmid instability in E.coli BL21? - (reply: 3)
581. Cleaving Fusion Proteins - (reply: 1)
582. Plyse cell and DH5a - (reply: 1)
583. Quality of aliquot for SDS-page in protein purification - When is pure pure enough? (reply: 1)
584. Electroelution with no electroeluter - (reply: 3)
585. reusing Ni NTA - (reply: 2)
586. Low expression: What else can I do? - Using pET23a in BL21 PlysS (reply: 9)
587. Changing dialysis buffer every half hour and then dialysis ON - protein precipitation prevention (reply: 1)
588. Checking For Protein Expression - (reply: 3)
589. How do I get rid of this contaminant? - His-tagged protein eluted with 300mM imidazole (reply: 13)
590. Is it possible? Harsh sonificiation conditions cause degradation of my protein - (reply: 3)
591. Low Thrombin Cleavage Efficiency Problem - I am trying to cleave a GST tagged protein(about 35 kDa + 26 kDa GST) (reply: 1)
592. Throbin cleavage declines with older protein? - It used to work, now doesn't! (reply: 1)
593. Couple questions about GST purification - (reply: 16)
594. Immunoprecipitation - (reply: 12)
595. Newbie question: how to determine protein concentration after IP? - (reply: 8)
596. protein extraction using trizol - can we replace guanidine cholride with other denaturants? (reply: 1)
597. High pressure on Ni-NTA beads - (reply: 2)
598. affinity column problems - (reply: 1)
599. Desalting protein - (reply: 7)
600. Trypsin Expression and Purification - (reply: 3)
572. membrane protein extraction buffer - (reply: 1)
573. What about the Baculovirus expression system? - (reply: 2)
574. Ni-NTA beads and dialysis tubing - Purification using nickel beads (reply: 4)
575. Concentrating Protein with centrifuge concentrator but 50% glycerol!!? - (reply: 2)
576. protein is degraded after purification - (reply: 2)
577. protein expression - Preparation of starter cultures (reply: 15)
578. Problem with Protein elution using GST sepharose 4B - (reply: 1)
579. how to isolate insoluble protein in bacterial induction? - (reply: 4)
580. Plasmid instability in E.coli BL21? - (reply: 3)
581. Cleaving Fusion Proteins - (reply: 1)
582. Plyse cell and DH5a - (reply: 1)
583. Quality of aliquot for SDS-page in protein purification - When is pure pure enough? (reply: 1)
584. Electroelution with no electroeluter - (reply: 3)
585. reusing Ni NTA - (reply: 2)
586. Low expression: What else can I do? - Using pET23a in BL21 PlysS (reply: 9)
587. Changing dialysis buffer every half hour and then dialysis ON - protein precipitation prevention (reply: 1)
588. Checking For Protein Expression - (reply: 3)
589. How do I get rid of this contaminant? - His-tagged protein eluted with 300mM imidazole (reply: 13)
590. Is it possible? Harsh sonificiation conditions cause degradation of my protein - (reply: 3)
591. Low Thrombin Cleavage Efficiency Problem - I am trying to cleave a GST tagged protein(about 35 kDa + 26 kDa GST) (reply: 1)
592. Throbin cleavage declines with older protein? - It used to work, now doesn't! (reply: 1)
593. Couple questions about GST purification - (reply: 16)
594. Immunoprecipitation - (reply: 12)
595. Newbie question: how to determine protein concentration after IP? - (reply: 8)
596. protein extraction using trizol - can we replace guanidine cholride with other denaturants? (reply: 1)
597. High pressure on Ni-NTA beads - (reply: 2)
598. affinity column problems - (reply: 1)
599. Desalting protein - (reply: 7)
600. Trypsin Expression and Purification - (reply: 3)