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361. Problem with Pierce GST spin purification column - (reply: 6)
362. HIS-tagged protein purification - (reply: 12)
363. how to elute "only" my recombinant protein - final elutes comes with non-specific proteins... (reply: 4)
364. His-tagged protein purification problem - (reply: 2)
365. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
366. Signal Peptides - To leave or not to leave (reply: 2)
367. Low expression of pET21a construct - (reply: 6)
368. GST-fusion protein degrades in-vivo - (reply: 3)
369. Inclusion body purification - (reply: 1)
370. help with protein elution - (reply: 1)
371. where are they on SDS-PAGE gel of Co-IPed complex??? - (reply: 1)
372. Active protein extract from corn - (reply: 2)
373. How to prepare high quality brain tissue lysate? - Will high salt lysis buffer be helpful? (reply: 1)
374. Reduced Glutathione affects thrombin activity? - (reply: 2)
375. You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)
376. Should I worry about endotoxin? - (reply: 4)
377. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
378. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
379. Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
380. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
381. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
382. Western Blot of Large Protein Help - (reply: 1)
383. how to make protein soluble? - protein solubility (reply: 4)
384. protein yield is very low after purification - protein purification (reply: 9)
385. FZR1 PROTEIN EXPRESSION - (reply: 1)
386. Membrane solubilisation - (reply: 2)
387. Heterologous protein extraction in yeast for enzymatic activity test - (reply: 5)
388. His tag protein purification - (reply: 7)
389. Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
390. Protein precipitating need help choosing suitable buffering system - (reply: 3)
362. HIS-tagged protein purification - (reply: 12)
363. how to elute "only" my recombinant protein - final elutes comes with non-specific proteins... (reply: 4)
364. His-tagged protein purification problem - (reply: 2)
365. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
366. Signal Peptides - To leave or not to leave (reply: 2)
367. Low expression of pET21a construct - (reply: 6)
368. GST-fusion protein degrades in-vivo - (reply: 3)
369. Inclusion body purification - (reply: 1)
370. help with protein elution - (reply: 1)
371. where are they on SDS-PAGE gel of Co-IPed complex??? - (reply: 1)
372. Active protein extract from corn - (reply: 2)
373. How to prepare high quality brain tissue lysate? - Will high salt lysis buffer be helpful? (reply: 1)
374. Reduced Glutathione affects thrombin activity? - (reply: 2)
375. You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)
376. Should I worry about endotoxin? - (reply: 4)
377. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
378. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
379. Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
380. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
381. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
382. Western Blot of Large Protein Help - (reply: 1)
383. how to make protein soluble? - protein solubility (reply: 4)
384. protein yield is very low after purification - protein purification (reply: 9)
385. FZR1 PROTEIN EXPRESSION - (reply: 1)
386. Membrane solubilisation - (reply: 2)
387. Heterologous protein extraction in yeast for enzymatic activity test - (reply: 5)
388. His tag protein purification - (reply: 7)
389. Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
390. Protein precipitating need help choosing suitable buffering system - (reply: 3)