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301. Protein Expression problems - (reply: 4)
302. Advice on soluble aggregate problem - (reply: 2)
303. problem in expressing A DNA binding protein - (reply: 6)
304. Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
305. histone proteins extraction and 2D - (reply: 1)
306. Nickel Column Purficiation - (reply: 2)
307. Sarkosyl and Ni-NTA - (reply: 1)
308. Insoluble Protein purification - (reply: 1)
309. Problems with detergents and Ni-NTA - (reply: 5)
310. Missing expression of proteins of interest in Pichia pastoris - (reply: 2)
311. Bradford method - (reply: 4)
312. protein extraction from pirmary keratinocytes - (reply: 2)
313. How to purify unstable protein? - (reply: 10)
314. unstable flag tagged protein - (reply: 4)
315. Hisx18 purification - (reply: 4)
316. Streptactin sepharose purification- binding problem - (reply: 2)
317. remove antibody from eluate in co-immunoprecipitation - (reply: 2)
318. How to obtain high-level production of low molecular weight recombinant proteins - (reply: 1)
319. Insoluble Protein - (reply: 5)
320. AKTA Injection Syringes - (reply: 3)
321. pDEST42 induction fail - (reply: 2)
322. getting rid of imidazole in purified protein? - (reply: 2)
323. crude enzyme precipitation is inclusion body? - (reply: 2)
324. Anion exchange purification - (reply: 3)
325. Expression of protein X varies in different w.b running (in one cell line, but - (reply: 3)
326. Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
327. Bacterial expression: incorporating radioactivity into overexpressed protein - (reply: 1)
328. a few questions about ion exchange chromatography - (reply: 31)
329. No activity of fungi protein in E.coli - (reply: 15)
330. Rehydrating/Resuspending Drosophila Proteins - (reply: 1)
302. Advice on soluble aggregate problem - (reply: 2)
303. problem in expressing A DNA binding protein - (reply: 6)
304. Immunoprecipitation---Why would a 250kDa band be present for the purification of - (reply: 8)
305. histone proteins extraction and 2D - (reply: 1)
306. Nickel Column Purficiation - (reply: 2)
307. Sarkosyl and Ni-NTA - (reply: 1)
308. Insoluble Protein purification - (reply: 1)
309. Problems with detergents and Ni-NTA - (reply: 5)
310. Missing expression of proteins of interest in Pichia pastoris - (reply: 2)
311. Bradford method - (reply: 4)
312. protein extraction from pirmary keratinocytes - (reply: 2)
313. How to purify unstable protein? - (reply: 10)
314. unstable flag tagged protein - (reply: 4)
315. Hisx18 purification - (reply: 4)
316. Streptactin sepharose purification- binding problem - (reply: 2)
317. remove antibody from eluate in co-immunoprecipitation - (reply: 2)
318. How to obtain high-level production of low molecular weight recombinant proteins - (reply: 1)
319. Insoluble Protein - (reply: 5)
320. AKTA Injection Syringes - (reply: 3)
321. pDEST42 induction fail - (reply: 2)
322. getting rid of imidazole in purified protein? - (reply: 2)
323. crude enzyme precipitation is inclusion body? - (reply: 2)
324. Anion exchange purification - (reply: 3)
325. Expression of protein X varies in different w.b running (in one cell line, but - (reply: 3)
326. Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
327. Bacterial expression: incorporating radioactivity into overexpressed protein - (reply: 1)
328. a few questions about ion exchange chromatography - (reply: 31)
329. No activity of fungi protein in E.coli - (reply: 15)
330. Rehydrating/Resuspending Drosophila Proteins - (reply: 1)