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481. How to concentrate the protein - (reply: 4)
482. protein overexpression - factors affecting overexpression (reply: 3)
483. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
484. e coli strain selection for protein expression - (reply: 2)
485. Problem clariflying/filtering insect cell lysate - (reply: 2)
486. Ni column changing colors - (reply: 2)
487. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
488. Problem with Histag purification - (reply: 1)
489. Freezing eluted His tag protein - (reply: 5)
490. Why Ni-NTA beads became clumped after incubation? - Intensive Clumped (reply: 2)
491. His-tag protein lost it's tag - (reply: 2)
492. AnionExchange problem: proteins do not bind - (reply: 12)
493. How much protein should be loaded to the column during the purificiation? - Is less more? ;) (reply: 1)
494. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
495. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
496. Centrifugal Filter Unit - Trying to get a higher concentration. (reply: 3)
497. IMPACT system purification problem - (reply: 1)
498. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
499. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
500. How to normalize my samples before I transfect my cells - (reply: 6)
501. FBS interference in SDS PAGE - (reply: 3)
502. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
503. What make voltage not going up for the 3rd step in running IEF? - (reply: 2)
504. Help...purify and concentrate protein for IEF - (reply: 1)
505. preference of N/C terminus for tagging - (reply: 2)
506. NI-NTA purification - (reply: 2)
507. Could a 30aa peptides(3×flag+6×his） be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)
508. Ampicillin Mess-up(Experiment in progress atm) - Experiment still valid? (reply: 1)
509. RNA immunoprecipitation...HELP! - (reply: 4)
510. Dialysis - A question about buffers (reply: 6)
482. protein overexpression - factors affecting overexpression (reply: 3)
483. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
484. e coli strain selection for protein expression - (reply: 2)
485. Problem clariflying/filtering insect cell lysate - (reply: 2)
486. Ni column changing colors - (reply: 2)
487. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
488. Problem with Histag purification - (reply: 1)
489. Freezing eluted His tag protein - (reply: 5)
490. Why Ni-NTA beads became clumped after incubation? - Intensive Clumped (reply: 2)
491. His-tag protein lost it's tag - (reply: 2)
492. AnionExchange problem: proteins do not bind - (reply: 12)
493. How much protein should be loaded to the column during the purificiation? - Is less more? ;) (reply: 1)
494. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
495. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
496. Centrifugal Filter Unit - Trying to get a higher concentration. (reply: 3)
497. IMPACT system purification problem - (reply: 1)
498. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
499. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
500. How to normalize my samples before I transfect my cells - (reply: 6)
501. FBS interference in SDS PAGE - (reply: 3)
502. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
503. What make voltage not going up for the 3rd step in running IEF? - (reply: 2)
504. Help...purify and concentrate protein for IEF - (reply: 1)
505. preference of N/C terminus for tagging - (reply: 2)
506. NI-NTA purification - (reply: 2)
507. Could a 30aa peptides(3×flag+6×his） be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)
508. Ampicillin Mess-up(Experiment in progress atm) - Experiment still valid? (reply: 1)
509. RNA immunoprecipitation...HELP! - (reply: 4)
510. Dialysis - A question about buffers (reply: 6)