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Virus Related Discussions
  1. How to concentrate retrovirus produced by Phoenix-Ampho cell - (reply: 4)
  2. Adenoviral purification by CsCl - Only cell pellet or supernatant too??? (reply: 5)
  3. retrovirus Phoenix transfection - Help help (reply: 2)
  4. virus copy number - how to calculate from mass (reply: 1)
  5. No Retrovirus using the Phoenix - Ampho cells - (reply: 1)
  6. Hepatitis B Virus - (reply: 2)
  7. adenovirus CsCl centrifugation - (reply: 2)
  8. ChIP for virus - (reply: 5)
  9. Large MSCV Retroviral Vector Cloning --- Help Needed! - (reply: 3)
  10. RT PCR of viral RNA segment - (reply: 1)
  11. non viral transfection - efficiency? (reply: 4)
  12. Retrovirus freezing - (reply: 2)
  13. Know good vector backbone for PT67? - Viral transduction of EGFP to epitherial cell line (reply: 1)
  14. how to extract DNA from adenovirus for template of PCR - (reply: 2)
  15. HEK293 for Adenovirus propagation - 293T, QB1 what's the difference? (reply: 1)
  16. Retroviral calcium phosphate transfection - How much DNA should I use?? (reply: 1)
  17. How to detect virus in organ? - (reply: 4)
  18. help with retroviral infection of MEFs - (reply: 5)
  19. After transfection and passaging I loose my virus - (reply: 4)
  20. Decontamination of virus during infection work - what % of bleach is suitable for viral decontamination and and how lon (reply: 3)
  21. interaction of viral protein with RNAi machinery - (reply: 1)
  22. viral titration - need an answer ASAP please!! (reply: 8)
  23. Retroviral vector cloning help - (reply: 6)
  24. How do Phoenix cells produce virus - (reply: 1)
  25. Is it true that a cell line cannot be infected with virus again once it was alre - virus infection (reply: 1)
  26. Proteinase K treatment on viral RNA - (reply: 3)
  27. How to infect T cells? Lentiviral or retroviral? - (reply: 2)
  28. who can introduce a book of virology? - (reply: 2)
  29. What would be the advantage of adenoviral transduction? - (reply: 2)
  30. Determining the RT concentration in HIV-1 samples through Sandwich ELISA - Not seeing any infection/RT activity/indication of virus (reply: 7)
  31. Using Adenovirus to transfect cells - (reply: 5)
  32. RECOMMEND VIROLOGY BOOK - ASK FOR RECOMMENDATION (reply: 2)
  33. recommendations for a viral titer cell line - (reply: 3)
  34. Determination of virus titer - (reply: 3)
  35. PCR from cell culture - to detect the presence of adenovirus (reply: 2)
  36. determination of virus concentration - empty viral concentration (reply: 4)
  37. Unknown Virus - Need help to identify virus (reply: 5)
  38. adenovirus purification - using CsCl (reply: 2)
  39. Trouble with viral vector --> too many colonies in control plate - Trouble with viral vector --> too many colonies in control plate (reply: 6)
  40. phoenix 293 retroviral infection - (reply: 4)
  41. Need large amounts of pure viral dna - (reply: 2)
  42. Sindbis virus expression - cannot detect EGFP signal in hippocampal neurons (reply: 1)
  43. finding virus molecular weight - (reply: 1)
  44. Please save me ! - pAdeasy adenovirus vector system (reply: 1)
  45. Adenoviral Transfection - cells to check transfection efficiency (reply: 4)
  46. Retroviral vector and recombination - Are Retroviral vector plasmids susceptible to recombination?why? (reply: 1)
  47. viral transduction AND lipofection of neurons - (reply: 1)
  48. About the adenovirus infection efficiency on tissue culture - (reply: 1)
  49. No virus titer and MSCV-mir30 vector problem - HELP (reply: 12)
  50. MSCV mir30 retroviral vector problem - (reply: 2)
  51. Simple method for concentrating adeno-associated virus(rAAV) - (reply: 6)
  52. Storage of virus at -80 - (reply: 7)
  53. reverse transcription from viral rna - (reply: 9)
  54. Retroviral vector - (reply: 3)
  55. retrovirus transfection in NIH3T3 - (reply: 3)
  56. Viral / phage promoter prediction - (reply: 1)
  57. Virus Detection - Tests for detecting specific virus (reply: 6)
  58. When are the cells virus free? - (reply: 6)
  59. question on virus infection efficiency - (reply: 2)
  60. Designing primer for Banana mild mosaic virus - (reply: 3)
  61. Expressing Viral glycoproteins in Mammalian cell-line? - (reply: 2)
  62. isoelectric point of japanase encephalitis virus - (reply: 1)
  63. Retroviral vs. Lentiviral packaging - Are the packaging signals compatible? (reply: 1)
  64. plasmids to quantify copies of virus - (reply: 4)
  65. Adenovirus Purification: CsCl solution sould be fresh? - Double CsCl Gradient (reply: 1)
  66. Viral RNA isolation from plant - (reply: 4)
  67. Viral RNA - have you ever run it in a gel? (reply: 6)
  68. ELISA with virus as antigen - (reply: 2)
  69. RT-PCR on 3kb fragment of viral RNA - (reply: 2)
  70. retrovirus secreted or not - (reply: 6)
  71. What molecular weight cutoff dialysis membrane to use for virus concentration - (reply: 11)
  72. HEPES and retroviral transduction - (reply: 4)
  73. looking for interferon titration kit - immunology/virology (reply: 6)
  74. Map of MIGR1 retroviral vector needed - (reply: 1)
  75. MIGR1 RETROVIRAL VECTOR - (reply: 2)
  76. pECL Eco viral vector and Rat cells - viral receptor present? (reply: 1)
  77. Titration of JEV - Plaque assay for Japanese encephalitis virus (reply: 1)
  78. who can compare adenoviral expression system and baculoviral expression system - (reply: 2)
  79. Overexpression of Viral Protein in mammalian cell line - (reply: 3)
  80. Question on adenovirus expression - (reply: 2)
  81. Harvesting virus - (reply: 10)
  82. Adenovirus Propagation - (reply: 2)
  83. difference between 293 and 293T for virus infection - (reply: 9)
  84. MIGR1 retroviral vector transfection - (reply: 3)
  85. transfection and adenovirus - probability of having adenovirus (reply: 11)
  86. How long for adenovirus CPE to show up after infection - (reply: 5)
  87. Amplify retroviral vectors doesn't work from miniprep to midiprep - (reply: 6)
  88. Yeast contamination in a adenoviral transfection - (reply: 3)
  89. Why do a nested PCR? (HIV virus) - (reply: 4)
  90. what determines wheather a virus can infect a host cell - (reply: 3)
  91. the way of viral RNA isolation from serum - (reply: 4)
  92. Virus Quantification Practical. - Questions about virus quantification (reply: 2)
  93. Retroviral transduction of cells - troubleshooting help please! - (reply: 8)
  94. Adeasy! - Adenovirus preparation (reply: 1)
  95. adenovirus vs. retrovirus cell infection--pros and cons? - infecting PC12 cells and overall general toxicity (reply: 3)
  96. Does it need cotransfection vector when retrovirus transfected hek293? - (reply: 7)
  97. HL-60 retrovirus infection - retrovirus infection system for HL-60 cells (reply: 5)
  98. Anion exchanger chromatography for virus purification - (reply: 1)
  99. pSM2C retrovirus vector from Openbiosystems, is it good? - (reply: 5)
  100. faster way to identify RNA virus other than RT-PCR? - (reply: 2)
  101. help! virus titter - (reply: 1)
  102. Tissue culture Infective dose - Infect the Human tumor ell culture by Newcastle virus (reply: 1)
  103. adenovirus purification - (reply: 1)
  104. virus culture and harvesting - (reply: 1)
  105. retroviral transfection of pheonix cells - (reply: 3)
  106. how to get highly concentrated adenovirus for in vivo mouse infection? - (reply: 1)
  107. Measuring the amount of virus! - How to calculate the virus concentration? (reply: 6)
  108. TCID50 - Tissue culture infective dose of virus. (reply: 1)
  109. Viral RT PCR - (reply: 2)
  110. Calculation of the 1.5 μmol of viral membrabe phospholipid - How to calculate the influenza virus concentration? (reply: 1)
  111. virus mutation rate monitor - (reply: 1)
  112. help with retrovirus production - have you ever produced retrovirus by yourself? (reply: 2)
  113. adenovirus infection: 293FT Vs 293A cells - low titer virus despite high CPE! (reply: 3)
  114. why HEK293 or HEK293T is used to produce retrovirus? - retrovirus problem! (reply: 4)
  115. retroviral vector - [help] pSM2c and LinX (reply: 2)
  116. Viral RNA detection - (reply: 1)
  117. Starting real time rt-pcr - working with rna virus (reply: 7)
  118. direct transfection vs. retroviral transfection - (reply: 2)
  119. Bird Flu Viral - (reply: 2)
  120. detection of viral mRNA help - (reply: 2)
  121. Irradiation of live virus vaccines - (reply: 4)
  122. bicistronic retroviral vector - Two both gene mammalian expression (reply: 2)
  123. Retroviral Vector won't digest ideas! - (reply: 1)
  124. stable eukaryotic transfection. - Neomycine resistance and bad viral titer (reply: 1)
  125. Role of MgCl2 in virus infection - (reply: 2)
  126. disrupted virus storage - disrupted 10A1 virus storage (reply: 1)
  127. Transfection of cells by viral DNA - (reply: 4)
  128. How to detect receptor on the cell surface? - using the less viral protein possible (reply: 1)
  129. General background question on virus DNA from a non-scientist - virus DNA (reply: 6)
  130. HELP: adenovirus transfection - (reply: 3)
  131. How to titer adenovirus stock - OD 260 vs. Plaque assay (reply: 1)
  132. Viral RNA extraction - Formalin fixed & Paraffin embended tissue (reply: 17)
  133. Adenovirus culture/isolation - (reply: 3)
  134. plasmid transfection and virus infection simutaneously - (reply: 2)
  135. qiagen column recycling - cleaning column of qiaamp viral rna mini kit (reply: 4)
  136. Retroviral transduction of mouse t lymphocytes - (reply: 2)
  137. Virus plaque assay for VSV - Protocol (reply: 1)
  138. First generation adenovirus Protocol - Protocol (reply: 1)
  139. how can i culture virus in primary cell lines? - microbiology (reply: 1)
  140. Human infection from Viral Vectors? - (reply: 4)
  141. PEG precipitation, what kinds of PEG I should use? - virology (reply: 2)
  142. Do all 293 cells contain an adenovirus? - Do all 293 cells contain an adenovirus? (reply: 3)
  143. virus vector for transfection - (reply: 8)
  144. Viral RNA & DNA extraction from serum - Protocols (reply: 1)
  145. Extraction of adenovirus DNA from cell culture for PCR - protocol (reply: 2)
  146. cloning of long viral fragment I - (reply: 2)
  147. virus purification - protocol (reply: 4)
  148. Separation of human DNA from virus DNA - (reply: 3)
  149. A question about adenovirus infection 293 cells - (reply: 5)
  150. Promoterless Retroviral vector? - (reply: 1)
  151. Largest viral insert? - How much DNA can be packaged? (reply: 1)
  152. Virus DNA - Where can i find purified virus DNA (reply: 2)
  153. Viral isolation - (reply: 1)
  154. Retroviral infection of primaryCD4+ T helper cells - (reply: 1)
  155. How to obtain VIRAL DNA frompurified virus prepation - (reply: 1)
  156. quantitative viral RNA assay - (reply: 1)
  157. retro virus titer - (reply: 5)
  158. adenovirus infection (293 cells)protocol - (reply: 1)