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Virus Related Discussions
How to concentrate retrovirus produced by Phoenix-Ampho cell -
(reply: 4)
Adenoviral purification by CsCl - Only cell pellet or supernatant too???
(reply: 5)
retrovirus Phoenix transfection - Help help
(reply: 2)
virus copy number - how to calculate from mass
(reply: 1)
No Retrovirus using the Phoenix - Ampho cells -
(reply: 1)
Hepatitis B Virus -
(reply: 2)
adenovirus CsCl centrifugation -
(reply: 2)
ChIP for virus -
(reply: 5)
Large MSCV Retroviral Vector Cloning --- Help Needed! -
(reply: 3)
RT PCR of viral RNA segment -
(reply: 1)
non viral transfection - efficiency?
(reply: 4)
Retrovirus freezing -
(reply: 2)
Know good vector backbone for PT67? - Viral transduction of EGFP to epitherial cell line
(reply: 1)
how to extract DNA from adenovirus for template of PCR -
(reply: 2)
HEK293 for Adenovirus propagation - 293T, QB1 what's the difference?
(reply: 1)
Retroviral calcium phosphate transfection - How much DNA should I use??
(reply: 1)
How to detect virus in organ? -
(reply: 4)
help with retroviral infection of MEFs -
(reply: 5)
After transfection and passaging I loose my virus -
(reply: 4)
Decontamination of virus during infection work - what % of bleach is suitable for viral decontamination and and how lon
(reply: 3)
interaction of viral protein with RNAi machinery -
(reply: 1)
viral titration - need an answer ASAP please!!
(reply: 8)
Retroviral vector cloning help -
(reply: 6)
How do Phoenix cells produce virus -
(reply: 1)
Is it true that a cell line cannot be infected with virus again once it was alre - virus infection
(reply: 1)
Proteinase K treatment on viral RNA -
(reply: 3)
How to infect T cells? Lentiviral or retroviral? -
(reply: 2)
who can introduce a book of virology? -
(reply: 2)
What would be the advantage of adenoviral transduction? -
(reply: 2)
Determining the RT concentration in HIV-1 samples through Sandwich ELISA - Not seeing any infection/RT activity/indication of virus
(reply: 7)
Using Adenovirus to transfect cells -
(reply: 5)
RECOMMEND VIROLOGY BOOK - ASK FOR RECOMMENDATION
(reply: 2)
recommendations for a viral titer cell line -
(reply: 3)
Determination of virus titer -
(reply: 3)
PCR from cell culture - to detect the presence of adenovirus
(reply: 2)
determination of virus concentration - empty viral concentration
(reply: 4)
Unknown Virus - Need help to identify virus
(reply: 5)
adenovirus purification - using CsCl
(reply: 2)
Trouble with viral vector --> too many colonies in control plate - Trouble with viral vector --> too many colonies in control plate
(reply: 6)
phoenix 293 retroviral infection -
(reply: 4)
Need large amounts of pure viral dna -
(reply: 2)
Sindbis virus expression - cannot detect EGFP signal in hippocampal neurons
(reply: 1)
finding virus molecular weight -
(reply: 1)
Please save me ! - pAdeasy adenovirus vector system
(reply: 1)
Adenoviral Transfection - cells to check transfection efficiency
(reply: 4)
Retroviral vector and recombination - Are Retroviral vector plasmids susceptible to recombination?why?
(reply: 1)
viral transduction AND lipofection of neurons -
(reply: 1)
About the adenovirus infection efficiency on tissue culture -
(reply: 1)
No virus titer and MSCV-mir30 vector problem - HELP
(reply: 12)
MSCV mir30 retroviral vector problem -
(reply: 2)
Simple method for concentrating adeno-associated virus(rAAV) -
(reply: 6)
Storage of virus at -80 -
(reply: 7)
reverse transcription from viral rna -
(reply: 9)
Retroviral vector -
(reply: 3)
retrovirus transfection in NIH3T3 -
(reply: 3)
Viral / phage promoter prediction -
(reply: 1)
Virus Detection - Tests for detecting specific virus
(reply: 6)
When are the cells virus free? -
(reply: 6)
question on virus infection efficiency -
(reply: 2)
Designing primer for Banana mild mosaic virus -
(reply: 3)
Expressing Viral glycoproteins in Mammalian cell-line? -
(reply: 2)
isoelectric point of japanase encephalitis virus -
(reply: 1)
Retroviral vs. Lentiviral packaging - Are the packaging signals compatible?
(reply: 1)
plasmids to quantify copies of virus -
(reply: 4)
Adenovirus Purification: CsCl solution sould be fresh? - Double CsCl Gradient
(reply: 1)
Viral RNA isolation from plant -
(reply: 4)
Viral RNA - have you ever run it in a gel?
(reply: 6)
ELISA with virus as antigen -
(reply: 2)
RT-PCR on 3kb fragment of viral RNA -
(reply: 2)
retrovirus secreted or not -
(reply: 6)
What molecular weight cutoff dialysis membrane to use for virus concentration -
(reply: 11)
HEPES and retroviral transduction -
(reply: 4)
looking for interferon titration kit - immunology/virology
(reply: 6)
Map of MIGR1 retroviral vector needed -
(reply: 1)
MIGR1 RETROVIRAL VECTOR -
(reply: 2)
pECL Eco viral vector and Rat cells - viral receptor present?
(reply: 1)
Titration of JEV - Plaque assay for Japanese encephalitis virus
(reply: 1)
who can compare adenoviral expression system and baculoviral expression system -
(reply: 2)
Overexpression of Viral Protein in mammalian cell line -
(reply: 3)
Question on adenovirus expression -
(reply: 2)
Harvesting virus -
(reply: 10)
Adenovirus Propagation -
(reply: 2)
difference between 293 and 293T for virus infection -
(reply: 9)
MIGR1 retroviral vector transfection -
(reply: 3)
transfection and adenovirus - probability of having adenovirus
(reply: 11)
How long for adenovirus CPE to show up after infection -
(reply: 5)
Amplify retroviral vectors doesn't work from miniprep to midiprep -
(reply: 6)
Yeast contamination in a adenoviral transfection -
(reply: 3)
Why do a nested PCR? (HIV virus) -
(reply: 4)
what determines wheather a virus can infect a host cell -
(reply: 3)
the way of viral RNA isolation from serum -
(reply: 4)
Virus Quantification Practical. - Questions about virus quantification
(reply: 2)
Retroviral transduction of cells - troubleshooting help please! -
(reply: 8)
Adeasy! - Adenovirus preparation
(reply: 1)
adenovirus vs. retrovirus cell infection--pros and cons? - infecting PC12 cells and overall general toxicity
(reply: 3)
Does it need cotransfection vector when retrovirus transfected hek293? -
(reply: 7)
HL-60 retrovirus infection - retrovirus infection system for HL-60 cells
(reply: 5)
Anion exchanger chromatography for virus purification -
(reply: 1)
pSM2C retrovirus vector from Openbiosystems, is it good? -
(reply: 5)
faster way to identify RNA virus other than RT-PCR? -
(reply: 2)
help! virus titter -
(reply: 1)
Tissue culture Infective dose - Infect the Human tumor ell culture by Newcastle virus
(reply: 1)
adenovirus purification -
(reply: 1)
virus culture and harvesting -
(reply: 1)
retroviral transfection of pheonix cells -
(reply: 3)
how to get highly concentrated adenovirus for in vivo mouse infection? -
(reply: 1)
Measuring the amount of virus! - How to calculate the virus concentration?
(reply: 6)
TCID50 - Tissue culture infective dose of virus.
(reply: 1)
Viral RT PCR -
(reply: 2)
Calculation of the 1.5 μmol of viral membrabe phospholipid - How to calculate the influenza virus concentration?
(reply: 1)
virus mutation rate monitor -
(reply: 1)
help with retrovirus production - have you ever produced retrovirus by yourself?
(reply: 2)
adenovirus infection: 293FT Vs 293A cells - low titer virus despite high CPE!
(reply: 3)
why HEK293 or HEK293T is used to produce retrovirus? - retrovirus problem!
(reply: 4)
retroviral vector - [help] pSM2c and LinX
(reply: 2)
Viral RNA detection -
(reply: 1)
Starting real time rt-pcr - working with rna virus
(reply: 7)
direct transfection vs. retroviral transfection -
(reply: 2)
Bird Flu Viral -
(reply: 2)
detection of viral mRNA help -
(reply: 2)
Irradiation of live virus vaccines -
(reply: 4)
bicistronic retroviral vector - Two both gene mammalian expression
(reply: 2)
Retroviral Vector won't digest ideas! -
(reply: 1)
stable eukaryotic transfection. - Neomycine resistance and bad viral titer
(reply: 1)
Role of MgCl2 in virus infection -
(reply: 2)
disrupted virus storage - disrupted 10A1 virus storage
(reply: 1)
Transfection of cells by viral DNA -
(reply: 4)
How to detect receptor on the cell surface? - using the less viral protein possible
(reply: 1)
General background question on virus DNA from a non-scientist - virus DNA
(reply: 6)
HELP: adenovirus transfection -
(reply: 3)
How to titer adenovirus stock - OD 260 vs. Plaque assay
(reply: 1)
Viral RNA extraction - Formalin fixed & Paraffin embended tissue
(reply: 17)
Adenovirus culture/isolation -
(reply: 3)
plasmid transfection and virus infection simutaneously -
(reply: 2)
qiagen column recycling - cleaning column of qiaamp viral rna mini kit
(reply: 4)
Retroviral transduction of mouse t lymphocytes -
(reply: 2)
Virus plaque assay for VSV - Protocol
(reply: 1)
First generation adenovirus Protocol - Protocol
(reply: 1)
how can i culture virus in primary cell lines? - microbiology
(reply: 1)
Human infection from Viral Vectors? -
(reply: 4)
PEG precipitation, what kinds of PEG I should use? - virology
(reply: 2)
Do all 293 cells contain an adenovirus? - Do all 293 cells contain an adenovirus?
(reply: 3)
virus vector for transfection -
(reply: 8)
Viral RNA & DNA extraction from serum - Protocols
(reply: 1)
Extraction of adenovirus DNA from cell culture for PCR - protocol
(reply: 2)
cloning of long viral fragment I -
(reply: 2)
virus purification - protocol
(reply: 4)
Separation of human DNA from virus DNA -
(reply: 3)
A question about adenovirus infection 293 cells -
(reply: 5)
Promoterless Retroviral vector? -
(reply: 1)
Largest viral insert? - How much DNA can be packaged?
(reply: 1)
Virus DNA - Where can i find purified virus DNA
(reply: 2)
Viral isolation -
(reply: 1)
Retroviral infection of primaryCD4+ T helper cells -
(reply: 1)
How to obtain VIRAL DNA frompurified virus prepation -
(reply: 1)
quantitative viral RNA assay -
(reply: 1)
retro virus titer -
(reply: 5)
adenovirus infection (293 cells)protocol -
(reply: 1)