retro virus titer - (Jun/17/2002 )

can someone tell me what is the best way to find aretrovirus titer when i dont have a lac z plasmid ?

-yaniv-

How about making your viruses to express EGFP, and then titer by flow cytometry?

-Emme Kay-

thank you very mach for your answer amme but i all ready got my constract and ineed to now how can i find avirus titer when i got no insaid marker
did you ever used areverse transcriptas assay kit ?

-yaniv-

If you are set up try in situ PCR to titre your virus.

Titering Replication-Defective Virus for Use in Gene Transfer

Strayer, D.S.; Duan, L.-X.; Ozaki, I.; Milano, J.; Bobraski, L.E.; Bagasra, O.
Published Date: 03/15/1997, Benchmarks, BioTechniques 22:447-450

-milanoj-

dear milanoj
thank you very muche for your advaise
i will realy aprishiate it if you coud give me aprotocol for this methods

-yaniv-

yaniv,
See above citation.

-milanoj-