ChIP for virus - (Oct/13/2008 )

Hi all,

I am currently planning an ChIP experiment for a virus-infected sample. I have some problems concerning the attenuation of the virus in the sample. In a typical ChIP experiment, it involves sonication to release the cellular extract. But since my sample is a virus-infected one, I will prefer to include a boiling step (for 10 min) to make sure the infectious viruses are attenuated. My question is, how will the protein-DNA mixture be after boiling? Will the protein be released from the DNA?

Thank you all very much!

Hi all,

I am currently planning an ChIP experiment for a virus-infected sample. I have some problems concerning the attenuation of the virus in the sample. In a typical ChIP experiment, it involves sonication to release the cellular extract. But since my sample is a virus-infected one, I will prefer to include a boiling step (for 10 min) to make sure the infectious viruses are attenuated. My question is, how will the protein-DNA mixture be after boiling? Will the protein be released from the DNA?

Thank you all very much!

Thank you very much!

I wonder if there is any methold to inactivate the viruses without causing protein denaturation.

I would guess so.

Oh, thank you very very much!