ASAP: ONE STEP Real time RT PCR kit for TWO step RT-PCR method? - (May/02/2006 )

hi all,
i am really in deep trouble. i had accidently ordered the wrong kit!! i am plannning to do TWO step RT PCR and i bought the ONE step RQ RT-PCR kit. i have synthesized all my cDNA and i don't it to be wasted and since the kit is very expensive!!
does anybody know can i use the ONE step kit for my work by excluding the Reverse transcription step? i will just proceed to the DNA amplification step of the PCR.
please let me know ASAP!!

thanks n regards,
katsumi

-katsumi-

QUOTE (katsumi @ May 3 2006, 01:33 AM)

Hi Katsumi

I've never done this but it should work. You are inactivating the RT Enzyme during the first denaturation of your PCR. The only problem could be that the buffer is over concentrated because it's designt for pure RNA and not for cDNA. But if you dilute the cDNA anyway and if you run serial dilutions of standards to check the efficienty of your reactions it chould be fine.

-Montgomery Burns-