house keeping gene not intron spanning and sample DNase UNtreated - (Sep/19/2008 )

Hi all,

is it possible to analyze the data I obtained from a sybr green qPCR where:

house keeping gene was not intron spanning while my genes of interest are exon spanning and they were checked in -RT control (gave no band).

I obtained the RNA using QIAGEN RNeasy columns but without DNase treatment.

My RNA as well as cDNA is finished and to repeat the procedure (get the cells) takes long... is there someone who would know how to make the result usable?

Could I assume that the possible DNA contamination is the same in my samples, since the number of cells used was comparable and thus the role of house keeping - to normalize for RNA concentration is not "damaged"?

thanks!

Hi all,

is it possible to analyze the data I obtained from a sybr green qPCR where:

house keeping gene was not intron spanning while my genes of interest are exon spanning and they were checked in -RT control (gave no band).

I obtained the RNA using QIAGEN RNeasy columns but without DNase treatment.

My RNA as well as cDNA is finished and to repeat the procedure (get the cells) takes long... is there someone who would know how to make the result usable?

Could I assume that the possible DNA contamination is the same in my samples, since the number of cells used was comparable and thus the role of house keeping - to normalize for RNA concentration is not "damaged"?

thanks!