Rotor-Gene 6000 - QPCR Machines (Sep/03/2008 )
First time Post, Long time reader...hehe.
I have a Rotor Gene 6000. I am trying to change the setting of the baseline. I cannot seem to find any way of doing this.
Any advice would be appreciated.
Thanks
-RatRace2000-
QUOTE (RatRace2000 @ Sep 3 2008, 04:11 PM)
First time Post, Long time reader...hehe.
I have a Rotor Gene 6000. I am trying to change the setting of the baseline. I cannot seem to find any way of doing this.
Any advice would be appreciated.
Thanks
I have a Rotor Gene 6000. I am trying to change the setting of the baseline. I cannot seem to find any way of doing this.
Any advice would be appreciated.
Thanks
I dont know if your question is already answerd, but really setting the baseline in the RG6000 is not possible, the software howeever has several features that are normally automaticly on when you do an analysis of your data (dynamic tube + slope correct).
Why do you want tot set your baseline?
-hebus-
I figured I could deal with problems I was having earlier with rainbows at the beginning of my reaction.
Speaking of which. I was able to determine that it was the tubes that were causing those early cycle anomalies. I contacted the company that makes the tubes and they don't really have any answer as to why or how.
-RatRace2000-
QUOTE (RatRace2000 @ Oct 9 2008, 08:12 PM)
I figured I could deal with problems I was having earlier with rainbows at the beginning of my reaction.
Speaking of which. I was able to determine that it was the tubes that were causing those early cycle anomalies. I contacted the company that makes the tubes and they don't really have any answer as to why or how.
Speaking of which. I was able to determine that it was the tubes that were causing those early cycle anomalies. I contacted the company that makes the tubes and they don't really have any answer as to why or how.
Those rainbows you see are quite normal, they do not interfere with the reaction, if they start to high however your treshold becomes higher and your reaction becomes less sensitive.
But in most cases this is no problem, just set your threshold above this rainbow and you will have no problems.
I have those rainbows to in every reaction, the stronger the reaction (the higher your signal goes), the less you see it.
One of my reactions is very robust and sensitve, it is not hold back by this artefact.
The only thing you can do is get your overal signal of your reaction higher. (more DNA, more primers, more probe,...
Sometimes less is more,...
-hebus-
QUOTE (RatRace2000 @ Oct 9 2008, 07:12 PM)
I figured I could deal with problems I was having earlier with rainbows at the beginning of my reaction.
Speaking of which. I was able to determine that it was the tubes that were causing those early cycle anomalies. I contacted the company that makes the tubes and they don't really have any answer as to why or how.
Speaking of which. I was able to determine that it was the tubes that were causing those early cycle anomalies. I contacted the company that makes the tubes and they don't really have any answer as to why or how.
Maybe a little late but:
You could see att your raw-data that these rainbow curves are not a correct positive one.
If your curve goes down in the beginning and then up after a while. The instrument can interpret it as a log curve.
You have a function that can disregard raw-data that not goes over X% over NTC, say that you put it to 5% all of you rainbow curves will probebly go away, surely at 10%. Read the manual about it and find out how it workes. It just a tool to use and you will not miss any "correct" curves.
-GrandLux-