Double tagging - (Apr/10/2012 )
Hi,
I have a protein which has both a GST fusion and a 6xHis tag. I was wodnering if I could just purify using either one of those tags (the his OR the GST) and still get good purified protein yield
thanks for your help
-LexyD-
it should be fine as long as the tag for which you select is exposed and accessible.
-mdfenko-