undesired products in multiplex PCR - (Aug/16/2010 )

I am studing with P. aeruginosa and I try to do multiplex PCR.I use 5 primer pairs. I have done MgCl and denturation temperature optimization. But ı have still problems about undesired products. also I have used DMSO in final consantration %9.There was no change in results. Please help m, ı am waiting your advises...

Try doing less primer pairs...

can you show your gel picture and your pcr protocols? if not is hard to advice.

Try to do as this paper said

Henegariu, O., N. A. Heerema, et al. (1997). "Multiplex PCR: critical parameters and step-by-step protocol." Biotechniques 23(3): 504-511.


I　think it will help you a lot