finding gene sequence to delete - (Feb/15/2013 )

Please if you can help.

My task is to find/locate a gene for oxoglutarate dehydrogenase (ketoglutarat dehydrogenase) in Streptomyces lividans or in Streptomyces coelicolor. I need a nucleotide sequence in order to delete it.

I have worked in human genetic lab till now and I am not familiar with this bacterial genome at all... Which page to use (I am used to ensembl for humans) to get a gene nt seq and locate it in bacterial genome?

Please help....

Hi,
Here is a link.

http://www.genome.jp/kegg-bin/get_htext?htext=br08601.keg&filedir=files&highlight=&option=&extend=&uploadfile=&format=&wrap=&length=&open=&close=&hier=&org_name=br08601&query=Streptomyces


In this link there is no Streptomyces lividans, but if the sequence is similar to the other streptomyces species in this link you can use that. I am working with E. coli. Usually we use different E. coli sequences. Mostly same genus carries almost similar sequences. I found your ketoglutarate dehydrogenase in Streptomyces coelicolor, 1272 aminoacids.

Best of luck,

http://www.genedb.org/Homepage/Scoelicolor

http://www.xbase.ac.uk/genome/streptomyces-lividans-tk24

Thanks Christy! You are my savior!

lmg

thanx neuron. ;-)

lmg