SOC vs TB in transformations - (Feb/26/2013 )
Hi all, wondering if its absolutely required to use SOC for transforming BL21, XL1 Blue and XL10 Gold cells. I tried it with TB and although I used TB for DH5alpha transformations before with no issues it didnt work here. Any ideas?
-LacquerHead-
SOC is an enriched medium containing glucose, while TB doesn't contain it. Some competent cells may be more sensitive to the initial growing conditions. And as I remember, XL10-Gold cells even require NZY+ broth for transformation (also containing glucose), however when I was doing mutagenesis I didn't have it and used SOC instead and got some colonies. But probably using the right medium would improve the transformation efficiency.
-Trof-