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RT-PCR Related Discussions
  1. RT-PCR for small RNAs - (reply: 1)
  2. What are normalisation primers for the rt-PCR? - Im going to order the mirVana rt-PCR kit for miRNAs (reply: 1)
  3. polyadenylation and RT-PCR - (reply: 2)
  4. Gene Expression level of apoptotic-related genes by qReal Time RT PCR - (reply: 1)
  5. MMLV RT - (reply: 2)
  6. -RT works better than +RT? - (reply: 9)
  7. RNA, cDNA, PCR - RT Inconsistency - High Quality RNA, Working RT, Varying PCR Results (reply: 2)
  8. RT-PCR works but not consistent - (reply: 3)
  9. Is Klenow necessary for RT-PCR? - (reply: 3)
  10. RT-PCR primer design - RT-PCR primer design...need ur help (reply: 3)
  11. RT-PCR primer design - RT-PCR primer design...need ur help (reply: 1)
  12. RT-PCR GAPDH Variability - (reply: 1)
  13. Help with RT-PCR of 1.4Kb fragment - (reply: 2)
  14. how to optimize rt-pcr for gene expression analysis - (reply: 3)
  15. genomic DNA contamination in RT-PCR - (reply: 7)
  16. how much cDNA do you use for RT-PCR? - (reply: 2)
  17. housekeeping gene in klebsiella pneumoniae for RT PCR - (reply: 2)
  18. RT PCR of viral RNA segment - (reply: 1)
  19. Do I need sterile RT-PCR tubes? - Real time PCR (reply: 5)
  20. wierd rt-pcr - (reply: 1)
  21. RT-PCR to amplify ORFs - (reply: 3)
  22. Transcript variation - measuring by RT-PCR - (reply: 3)
  23. when to set up a RT-PCR for mRNA expression? - detect transcription of transgene (reply: 1)
  24. When is the right time to adjust RNA concentrations for Rt-PCR? - (reply: 2)
  25. checking mRNA expression by reverse transcriptase PCR - (reply: 2)
  26. Inconsistent Real Time results- RT efficiency, high Ct values - (reply: 3)
  27. Best house keeping gene for RT-PCR - (reply: 6)
  28. RT-PCR inconsistant results - (reply: 1)
  29. Standard Curve method RT PCR- - (reply: 2)
  30. RT-PCR reagents KIT 1 or 2-step? - (reply: 3)
  31. How to get GOOD rt-pcr bands - (reply: 7)
  32. Choice of one-step or two-steps RT-PCR - (reply: 3)
  33. RT-PCR primer design and in silico PCR - (reply: 4)
  34. Checking mRNA expression by RT-PCR - (reply: 2)
  35. RT-PCR no amplification - (reply: 2)
  36. quadraplex RT-PCR error - (reply: 1)
  37. Internal Standards for RT-pCR - (reply: 11)
  38. Can a 96 well plate for rt PCR be used for ELISA? - 96 well plate , ELISA (reply: 2)
  39. Questions about PCR amplification using cDNA (RT from total RNA). - (reply: 6)
  40. RT-PCR from VERY limited quantity of tissue sample - Need suggestion on RNA isolation and RT kits to maximize cDNA yield (reply: 1)
  41. Total RNA isolation for RT-PCR - (reply: 3)
  42. Failed RT-PCR amplification of 3-&5''-ligated small RNAs - (reply: 5)
  43. RT-PCR for various sample with Tm from 45-60 degree - (reply: 1)
  44. cDNA quantification by non-PCR method - measuring RT efficiency (reply: 1)
  45. Double Stranded cDNA Low Yields - DS cDNA RT Yield Troubleshooting (reply: 2)
  46. Help -designing primers for real time RT-PCR - (reply: 1)
  47. Inefficient RT-PCR, ¿bad cDNA? - don't know why (reply: 1)
  48. help with RT-PCR - DNase usage - (reply: 2)
  49. A couple of (RT-PCR) cowboy questions - I feel the need, the need for speed (reply: 2)
  50. Getting primer dimers in RT-PCR on and off? - (reply: 2)
  51. Internal control for RT-PCR analysis - The expression of internal control (reply: 3)
  52. What are different ways of genotyping a transgenic mouse? - what is best way to do genotyping--PCR, qRT-PCR or RT-PCR (reply: 3)
  53. free programme for RT-PCR quantification - free programme for RT-PCR quantification (reply: 3)
  54. 1:10 serial dilution in rt-pcr with slope of -0.58 ... - (reply: 1)
  55. Cell lysate at RT - (reply: 4)
  56. RT-PCR or disulfite treatment, which is better? - (reply: 5)
  57. Starting with semi quantitative RT-PCR and a little bit lost.. - (reply: 4)
  58. RNA extraction from laser capture microdissections - how to see low expressed genes from trought RT/qPCR on laser capture m (reply: 2)
  59. cDNA stability after miRNA stem-loop RT - (reply: 2)
  60. Housekeeping genes for RT-PCR in soybean - (reply: 6)
  61. Anchored oligo dT and Thermostable reverse transcriptase - temperature compatibility between RT and oligo dT priming (reply: 8)
  62. Stem-loop RT primers: how to anneal? - (reply: 1)
  63. RT PCR for methylation analysis - (reply: 3)
  64. RT-PCR cloning: oligo dT-primed cDNA synthesis or anchored? - (reply: 1)
  65. RT-PCR Vs qRT-PCR help please! - (reply: 4)
  66. Primers from RT-PCR database - RT-PCRdatabase primers giving primer dimers (reply: 1)
  67. minimum mRNA concentration for RT-PCR - (reply: 4)
  68. Troubleshooting RT-PCR - (reply: 7)
  69. Thermostable Reverse Transcriptase for RT-PCR - (reply: 3)
  70. Can I run q RT real-time PCR based on cDNA amount? - (reply: 1)
  71. RT-PCR consistency - (reply: 2)
  72. antibody incubation on western blots - difference between 4°C and RT (reply: 10)
  73. New to rt pcr real time - guidelines and advice (reply: 3)
  74. Statistics: real-time RT-PCR - (reply: 4)
  75. RT-PCR using formaldehyde cross linked samples - RNA extraction using CHIP crosslinked samples (reply: 1)
  76. RT-Pcr without RNA isolation? - (reply: 3)
  77. RT PCR Standard curve amplification problem - (reply: 1)
  78. Determining the RT concentration in HIV-1 samples through Sandwich ELISA - Not seeing any infection/RT activity/indication of virus (reply: 7)
  79. The best reverse transcriptase for RT PCR - (reply: 5)
  80. What is the suitable product size for conventional RT-PCR analysis - products with size over 1 kb? (reply: 6)
  81. weak RT-PCR product - (reply: 2)
  82. puzzling RT question - (reply: 4)
  83. 10 fold dilutions show no RT-PCR product whatsoever - (reply: 1)
  84. RT-PCR - necessary to run electrophoresis gels? - (reply: 4)
  85. RNA handling for RT-PCR? - (reply: 4)
  86. RT PCR Primer dimer tissue dependent SYBR - (reply: 1)
  87. Can we compare traditional RT-PCR results with Real Time RT-PCR? - (reply: 1)
  88. any problems with RT-PCR for large amplicon sizes? - very strange amplification curves (reply: 2)
  89. HIV RT activity assay kit - (reply: 1)
  90. problem rt pcr long gene - (reply: 4)
  91. TNES Urea - keep at RT or 4 C? (reply: 2)
  92. RT PCR produces nice crisp band but of wrong length :-) - (reply: 2)
  93. RT-PCR gene specific amplification problem - (reply: 1)
  94. RT-PCR: Band in no reverse transcriptase lane - (reply: 18)
  95. Non repeatability of RT-PCR with same cDNA - (reply: 3)
  96. Keep RT mix on ice for 4 hrs - RT-PCR (reply: 3)
  97. How get GFP signal without mRNA target from pIRES-EGFP - positive by FACS but no DNA amplifation from RT-PCR (reply: 8)
  98. 2-step RT-PCR - (reply: 1)
  99. RT-PCR NTC- substitute DNA with water? - or just end up with a smaller rxn volume? (reply: 1)
  100. RT-PCR Controls (no DNA) show weird amplification - Complicated problem- urgent help needed! (reply: 4)
  101. RT-PCR primer designing - (reply: 6)
  102. Reverse Transcriptase PCR - Step by step protocol (reply: 6)
  103. Problem about RT-PCR for beta-actin - (reply: 2)
  104. About Reverse transcriptase PCR followed by PCR - primers (reply: 1)
  105. autoclaving tubes for PCR/RT-PCR - (reply: 5)
  106. RT PCR - NO PCR PRODUCT (reply: 3)
  107. RT PCR strange melting curve - (reply: 3)
  108. RNA quantification for Real Time RT-PCR - (reply: 2)
  109. RT-PCR without RNA isolation - (reply: 2)
  110. Does non-digested RNA in cDNA sample have an effect on RT-PCR quantification? - (reply: 2)
  111. Other than Taq? - for PCR after RT (reply: 3)
  112. multiplex rt pcr - (reply: 5)
  113. Knockdown detected by western but not RT-PCR - (reply: 3)
  114. minimum amount of rna in RT-PCR - (reply: 1)
  115. multiplex RT-PCR - (reply: 6)
  116. oligos as synthetic "miRNA" for quantification? - would an oligo / primer work in AB RT kit? (reply: 2)
  117. DNase digestion step necessary for real-time RT-PCR? - (reply: 2)
  118. improve the DNA cons. in RT-PCR - (reply: 2)
  119. primer for real-time and reverse transcriptase PCR - (reply: 1)
  120. ask for help about the RT-PCR - does different aoumnt of RNA affect the RT efficiency? (reply: 1)
  121. RT-PCR not working - (reply: 8)
  122. Representing RT-PCR Data - (reply: 2)
  123. RT-PCR Question.. - (reply: 1)
  124. RT-PCR kit - (reply: 3)
  125. condition for keeping integrity of RNA for RT-PCR - (reply: 1)
  126. Amplified Minus RT - (reply: 1)
  127. RT-PCR Troubles.. - (reply: 3)
  128. pcr product afetr reverse transcriptase reaction - 4 bands i have not included the band which i want??? (reply: 5)
  129. Help! Jesus, I left my postdoc's samples out in RT for 5 hours....... - (reply: 7)
  130. Problem - same ct values in my RT positive and negative samples - (reply: 3)
  131. RT-PCR product dilution and electrophoresis - (reply: 2)
  132. DNase treatment of RNA prior to RT-PCR - (reply: 4)
  133. RT PCR for larger amplicon - (reply: 2)
  134. one Step RT with Sybr and standard curve - (reply: 1)
  135. RT-PCR on 3kb fragment of viral RNA - (reply: 2)
  136. can Primers for RT-PCR be used Real-Time PCR directly? - (reply: 2)
  137. Silly Question about RT-PCR/Primer design - (reply: 2)
  138. primer selection for RT prior to real time - an insiders hint!!! (reply: 1)
  139. Which primer concentration for RT with very low RNA amounts? - (reply: 1)
  140. loading control of RT-PCR - (reply: 3)
  141. RT-PCR primer design: Over introns and exons? - (reply: 2)
  142. RT-PCR - not qPCR! urgent please - how to choose primers (reply: 3)
  143. RT-PCR primers - (reply: 3)
  144. RT control product appears only 5 cycles away from unknown samples - (reply: 2)
  145. Which result is correct-Western or RT-PCR - (reply: 5)
  146. rt-pcr data and western blot data not compatible - what could be the explanation (reply: 5)
  147. RT-PCR of apoptosis related genes - (reply: 2)
  148. RT PCR Primer - Software (reply: 2)
  149. RT-PCR problems on mouse ear RNA - (reply: 1)
  150. RT-PCR primer design - (reply: 1)
  151. strange RT-PCR expression - (reply: 1)
  152. Problem with cloning p53-suggestions for RT-PCR - (reply: 1)
  153. RT-PCR control for Vitis vinifera - (reply: 2)
  154. oligo(dT) plus random primers in RT - (reply: 4)
  155. no RT controls - can I run on separate plate and for just one primer set? (reply: 1)
  156. Relative Quantification in real-time RT-PCR - (reply: 5)
  157. Whether Mg2+ can be released from the Reverse Transcriptase after Its Inactivati - Urgent (reply: 3)
  158. No RT control showed strange results - (reply: 3)
  159. Any one can tell me what happens to my RT-PCR? - Urgent !RT-PCR really kills me! (reply: 5)
  160. NTC's in RT or just qPCR? - (reply: 3)
  161. qPCR, no RT controls always positive (as cDNA samples) - (reply: 4)
  162. faster way to identify RNA virus other than RT-PCR? - (reply: 2)
  163. RT-PCR a GC Rich (68%) gene (2200bp) - (reply: 6)
  164. Two bands on RT-PCR product - (reply: 8)
  165. Stem loop RT-PCR for miRNA quantification - how can I design the stem loop primer? (reply: 2)
  166. ASAP: ONE STEP Real time RT PCR kit for TWO step RT-PCR method? - (reply: 1)
  167. RT-PCR mouse RNA released from complement-lysed cells - Goal: to whether RNA is of nuclear or mitochondrial origin. Planning to PCR ampl (reply: 2)
  168. choice of reverse transcriptase - (reply: 4)
  169. Validating microarray results - Real-time RT-PCR (reply: 1)
  170. Best product size for real-time RT-PCR - (reply: 4)
  171. RT PCR on ALM patients - help for designing primers for detect PML RAR (reply: 2)
  172. Viral RT PCR - (reply: 2)
  173. Reverse Transcriptase PCR - why radiolabeling? (reply: 4)
  174. RT-PCR machine/setup problems - (reply: 2)
  175. RT-PCR, DNA contaminant removal by digestion with restriction enzyme - (reply: 3)
  176. RT-PCR question - genomic DNA amplified with RNA (reply: 9)
  177. RT-PCR Control Reactions - Need suggestions! - (reply: 1)
  178. Long RT for 4.5 kb cDNA - (reply: 5)
  179. RT issues and efficiency of cDNA synthesis - qRT-PCR (reply: 1)
  180. RNA storing/RT PCR using oligo dT - (reply: 5)
  181. stability of TOPO vector at RT - (reply: 3)
  182. HIV RT PCR KIT - (reply: 1)
  183. quantity of RNA to use in RT reaction - (reply: 3)
  184. Housekeeping gene for RT-PCR on plant - RT PCR (reply: 1)
  185. RT-PCR of an intron - possible plateau effect? (reply: 2)
  186. RT-PCR primer design at 3'-UTR or ORF? - (reply: 2)
  187. Smeary bands in real-time RT-PCR - (reply: 7)
  188. RT PCR - (reply: 1)
  189. Titration of Mg2+ in RT-PCR - (reply: 3)
  190. RT-PCR primers for E. coli LacZ gene - (reply: 3)
  191. RT-PCR amplification of an intron - has anyone tried this? (reply: 4)
  192. Picking stable clones by RT-PCR.. - (reply: 2)
  193. DTT & RNase inhibitor in RT reaction - (reply: 6)
  194. RT-PCR with an old DNaseI? - (reply: 3)
  195. positive control for RT PCR - (reply: 2)
  196. How can I get the 4.5kb Human typeI procollagen alpha1 chain cDNA Via RT-PCR fr - Seeking Help (reply: 14)
  197. More RT PCR questions: - can I amplify "everything?" (reply: 2)
  198. RT-PCR on plasmid - (reply: 1)
  199. Reverse transcriptase PCR - RTPCT (reply: 2)
  200. RT-PCR detection limits - (reply: 1)
  201. RT-PCR Primer design - which region? - (reply: 2)
  202. Cloning using RT-PCR for long fragment of 6kb - (reply: 1)
  203. RNA extraction with TRizol and RT-PCR - (reply: 8)
  204. Does Taq have reverse transcriptase activity? - (reply: 20)
  205. Sybr RT PCR problem - (reply: 4)
  206. RT-PCR for large fragment - (reply: 7)
  207. real time RT-PCR in bacteria - quantitative PCR (reply: 2)
  208. RT-PCR problem - negative control (reply: 1)
  209. Inconsistent RT-PCR results - (reply: 1)
  210. RT-PCR scale-down makes more sensitive? - (reply: 3)
  211. RT-PCR inconsistent primer efficiencies - RT-PCR (reply: 3)
  212. how much total RNA do you use for RT-PCR - (reply: 6)
  213. One Step RT-PCR cloning problem - (reply: 2)
  214. What kind of statistic methods? - RT-PCR (reply: 2)
  215. Primers for RT reaction - (reply: 6)
  216. Internal Control RNA & RT-PCR - (reply: 1)
  217. rt-PCR false postitives - (reply: 2)
  218. RT-PCR some questions - (reply: 2)
  219. Reamplification of RT-PCR product - (reply: 2)
  220. internal control in semi quantitative rt pcr - question (reply: 2)
  221. Which RT primer is better for qPCR - (reply: 2)
  222. Storage of RT-PCR products - (reply: 2)
  223. can't get rid of DNA contamination even with DNase for RT-PCR - (reply: 3)
  224. real-time RT-PCR one cell - (reply: 2)
  225. mutilple bands after RT-PCR - (reply: 2)
  226. RT-PCR and RE cut site-will this work? - (reply: 3)
  227. invitrogen Superscript one step RT PCR kit - Efficiency of kit (reply: 4)
  228. cDNA synthesis - which transcriptase is better? - (reply: 2)
  229. tRNA and Real time RT-PCR - (reply: 1)
  230. 3 bands on RT-PCR - (reply: 4)
  231. DNaseI have affect with RT-PCR or not - (reply: 3)
  232. RT- PCR? - (reply: 2)
  233. RT-PCR for an intronless gene - (reply: 6)
  234. High fidelity RT-PCR enzymes - looking for choices... (reply: 5)
  235. Problem with DNase treatment of RNA for RT-PCR - (reply: 1)
  236. realtime RT-PCR/internal control - (reply: 3)
  237. Realtime RT-PCR problem - no product - (reply: 6)
  238. RT-PCR gone bad - (reply: 4)
  239. RT-PCR - a lot of wrong size fragments. - (reply: 2)
  240. how to produce a high-quality cDNA template for RT-PCR - produce a high-quality cDNA template (reply: 4)
  241. RT-PCR - (reply: 1)
  242. Why is DTT used in reverse transcription (RT) reaction? - (reply: 9)
  243. RT-PCR - 2 kb fragment in RT-PCR (reply: 2)
  244. RT-PCR - (reply: 4)
  245. RT Question using superscript III RT - How many units per 20ul rxn of RT? (reply: 1)
  246. RT-PCR with B-cells as template - (reply: 1)
  247. RT-PCR of different cell lines - (reply: 1)
  248. primer design: end-point vs. RT - (reply: 1)
  249. RT-PCR primer design - exon-exon primer design (reply: 5)
  250. RT problem !! - (reply: 1)
  251. RT-PCR for cloning low expressed genes - RT-PCR for cloning low expressed genes (reply: 1)
  252. PCR or RT-PCR? - (reply: 3)
  253. Weak RT-PCR products? - (reply: 1)
  254. RT PCR - dilution prior to PCR (reply: 2)
  255. RT product check - Can I qualify my cDNA? (reply: 2)
  256. RT-PCR, why two PCR steps? - two PCRs after reverse transcription?? (reply: 2)
  257. total rna extraction from whole blood - for rt pcr (reply: 3)
  258. Trouble shooting RT-PCR - (reply: 4)
  259. RNA isolation from mouse blood - RNA for RT-PCR (reply: 1)
  260. Which gene is the best internal control for RT-PCR - (reply: 5)
  261. PCR primers - PCR primers for RT-PCR (reply: 1)
  262. RT-PCR;Northern-blotting - internal standard (reply: 4)
  263. correct bands in RT-PCR negative control - (reply: 14)
  264. RT-PCR, RNA isolation, etc. - (reply: 1)
  265. RT-PCR amplification problem - amplification problem (reply: 1)
  266. RT-PCR primer design guide - How to check gene structure and design the primer? (reply: 15)
  267. RT-PCR 1 step - (reply: 2)
  268. Detection in SmartCycler RT-PCR - (reply: 1)
  269. gDNA contamination in RT-PCR - (reply: 2)
  270. Help! DNA contamination in RNA and RT-PCR. - (reply: 1)
  271. oocyte RT - (reply: 3)
  272. Primer design - Primer design for RT-PCR (reply: 1)
  273. Difference between RNase Protention and RT-PCR - (reply: 1)
  274. RT-PCR - (reply: 2)
  275. degenerated primer for nested RT PCR - (reply: 5)
  276. RT-PCR - (reply: 1)
  277. RT-PCR - (reply: 1)
  278. RT PCR - (reply: 1)
  279. Difference in RT PCR products when using total RNA or mRNA - (reply: 4)
  280. RT-PCR, help needed - (reply: 2)
  281. Rt-PCR help,come in - use RT (reply: 2)
  282. RNA isolation from isolatedislets and RT-PCR - (reply: 1)
  283. RT-PCR - (reply: 2)
  284. problem with Rt-PCR - (reply: 1)
  285. Single-cell RT-PCR with only ~10-100 Cells - (reply: 2)
  286. C-MYC AND CYCLIN D1  / RT-PCR - (reply: 2)
  287. a question about RT-PCR - (reply: 1)