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RT-PCR for cloning low expressed genes - RT-PCR for cloning low expressed genes (Sep/30/2004 )

For cloning low expressed genes by RT-PCR, what is better: RT-PCR with a big amount of tRNA (25ug tRNA/ 20ul reaction) or low amount of tRNA (1ug/20ul)???

-mfpa-

Why use tRNA in RT-PCR?

-kawaka-