Validating microarray results - Real-time RT-PCR (Apr/03/2006 )
Hi there,
I have just did my real-time RT-PCR to validate the microarray results. I have obtained the fold-change of 10 genes from both methods, however, how do i compare them statistically? For instance, how can i obtain the R value? Usually what is the way to go about comparing the results?
Smiles,
Light_my_way
-light_my_way-
hi,
i m comparing interms of fold change between contorl and treatment.
i got almost same fold (set control as 1 and treatment in respect to it) difference in genechip and RT-PCR results.
gud luk
QUOTE (light_my_way @ Apr 3 2006, 11:44 PM)
Hi there,
I have just did my real-time RT-PCR to validate the microarray results. I have obtained the fold-change of 10 genes from both methods, however, how do i compare them statistically? For instance, how can i obtain the R value? Usually what is the way to go about comparing the results?
Smiles,
Light_my_way
I have just did my real-time RT-PCR to validate the microarray results. I have obtained the fold-change of 10 genes from both methods, however, how do i compare them statistically? For instance, how can i obtain the R value? Usually what is the way to go about comparing the results?
Smiles,
Light_my_way
-payeli-