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Validating microarray results - Real-time RT-PCR (Apr/03/2006 )

Hi there,

I have just did my real-time RT-PCR to validate the microarray results. I have obtained the fold-change of 10 genes from both methods, however, how do i compare them statistically? For instance, how can i obtain the R value? Usually what is the way to go about comparing the results?

Smiles,
Light_my_way

-light_my_way-

hi,
i m comparing interms of fold change between contorl and treatment.
i got almost same fold (set control as 1 and treatment in respect to it) difference in genechip and RT-PCR results.

gud luk

QUOTE (light_my_way @ Apr 3 2006, 11:44 PM)
Hi there,

I have just did my real-time RT-PCR to validate the microarray results. I have obtained the fold-change of 10 genes from both methods, however, how do i compare them statistically? For instance, how can i obtain the R value? Usually what is the way to go about comparing the results?

Smiles,
Light_my_way

-payeli-