RT-PCR with B-cells as template - (Nov/27/2004 )

Dear everyone,

I am trying to make cDNA directly from B-cells of immunized mouse as template. But, i didn't work. Does anyone know how to solve the problem

Thank

Hi Ali,

Hard to answer this question without any more specific information regarding what you've tried.

Also, I think you should re-post this question in the molecular biology section, rather than the immunology section. you'll get more answers that way...