Do protein purification under partially denatured condition - (Oct/24/2013 )
Hello, everyone,
I am wondering if there is anyone here has experience with purification under partially denatured condition, like add 2-4M Urea?
I want some support about this. Could you give me some materials about this, or some kind of web link?
Thank you very much.
-Hellen-
Dear Hellen,
Not sure if this helps, but there is a handbook for "protein sample preparation" which you can download from link below:
On top there are a few handbooks which might be beneficial as a reference for your research work as well.
Hope this helps.
Rgs,
Adrian
-Adrian K-