5' end RNA biotin labeling - need protocol (Apr/05/2009 )
hi all,
I need a protocol for biotinylation of 5' end of RNA. all I found was the protocols of labeling with 32P. 
-rimal-
Rimal,
This may be a bit crude, and there may be better methods, but if you're not too concerned about how many biotin molecules attach to each 5' end, you could extend the RNA with a limiting amount of biotinylated nucleotides.
-Carlton
-Carlton H-
Carlton H on Apr 5 2009, 05:44 PM said:
Rimal,
This may be a bit crude, and there may be better methods, but if you're not too concerned about how many biotin molecules attach to each 5' end, you could extend the RNA with a limiting amount of biotinylated nucleotides.
-Carlton
This may be a bit crude, and there may be better methods, but if you're not too concerned about how many biotin molecules attach to each 5' end, you could extend the RNA with a limiting amount of biotinylated nucleotides.
-Carlton
Can you explain more?
-rimal-