Yeast complementation test - (Apr/05/2009 )
Hi,
On a project that I have been working on is where I have created point mutations in cdc21 to look at how the mutations would affect the phosphorylation. After creating mutations and cloning the mutants to a suitable vector I need to study the complementation of temperature sensitivity of the mutated cdc21s to regular cdc21 /MCM4 . I’m confused about the concept of complementation and how I should get about doing the complementation tests using the regular cdc21 and the mutated cdc21. Further I have created my mutations but I'm not sure as to how I should pick a vector for expression. I'm new to this field and any help is greatly appreciated.
Thanks,
-needhelp-
needhelp on Apr 5 2009, 03:29 PM said:
Hi,
On a project that I have been working on is where I have created point mutations in cdc21 to look at how the mutations would affect the phosphorylation. After creating mutations and cloning the mutants to a suitable vector I need to study the complementation of temperature sensitivity of the mutated cdc21s to regular cdc21 /MCM4 . I’m confused about the concept of complementation and how I should get about doing the complementation tests using the regular cdc21 and the mutated cdc21. Further I have created my mutations but I'm not sure as to how I should pick a vector for expression. I'm new to this field and any help is greatly appreciated.
Thanks,
On a project that I have been working on is where I have created point mutations in cdc21 to look at how the mutations would affect the phosphorylation. After creating mutations and cloning the mutants to a suitable vector I need to study the complementation of temperature sensitivity of the mutated cdc21s to regular cdc21 /MCM4 . I’m confused about the concept of complementation and how I should get about doing the complementation tests using the regular cdc21 and the mutated cdc21. Further I have created my mutations but I'm not sure as to how I should pick a vector for expression. I'm new to this field and any help is greatly appreciated.
Thanks,
I think you should post your question in the subforum for protein expression. You'd get more response there.
-chrisbelle-