no band in gel - gel electrophoresis (May/06/2009 )

Hi all,
In real Time PCR, HIV RNA is positive. But in gel electrophoresis, no band is found. Why it is caused?
thanks,
thinsu :wacko:

-thinsu-

thinsu on May 6 2009, 12:52 PM said:

Hi all,
In real Time PCR, HIV RNA is positive. But in gel electrophoresis, no band is found. Why it is caused?
thanks,
thinsu :wacko:

What's your Ct? your elecrophoresis system might not be sensitive enough. You can try staining the gel after running for longer (with EtBr for ~15min then wash with water), or loading more sample.

-almost a doctor-