RNA extraction from blood stain - (Jan/16/2012 )
Hello everybody ,
I've purchased EZ-10 Spin Column Blood RNA Mini-Preps Kit to extract RNA from blood sample. Now I need to extract RNA from blood stain ( dried blood ). The kit only gives procedures to extract RNA from fresh blood sample, how can I use it to extract RNA from blood stain ?
you can find the data sheet of the kit at the following link :
http://www.biobasic.com/product/pdf/Information/info.BS82313-V6.0-.pdf
Thank you !
-Yahia Farid-
RNA is unstable and tends to degrade much faster than DNA. I think you can extract RNA from the dried blood sample by using 15 % methanol.
_________________________
information on dna extraction
-henryn-