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Sequencing Related Discussions
  1. sequencing problem - (reply: 2)
  2. PCR sequencing/cloning-seq for SNP identification - (reply: 1)
  3. dideoxy chain-termination method for sequencing DNA - (reply: 5)
  4. Advantages of ChIP vs. sodium bisulfite sequencing - (reply: 2)
  5. Bisulfite sequencing reproducibility - overlapping PCR amp primers give varying results (reply: 2)
  6. Why DNA sequencing does not detect deletions - help! - Med student lost on her hw. Breast cancer gene (BRCA1) mutations (reply: 3)
  7. Sequencing non-existent DNA? - (reply: 9)
  8. dna sequencing question - basic question (reply: 1)
  9. wierd dna sequencing results - any clues? (reply: 4)
  10. DNA Sequencing Problem - (reply: 6)
  11. Standard sequencing primers - how to find which sequencing primers bind to my DNA? (reply: 2)
  12. Direct genomic sequencing - (reply: 4)
  13. Comparative Genomics, Protein Sequencing, more - (reply: 1)
  14. dna sequencing problem - (reply: 8)
  15. colony screening by sequencing - (reply: 4)
  16. Does RNAase hinder sequencing reaction - (reply: 2)
  17. DNA sequencing downstream work - (reply: 3)
  18. Sequencing a stretch of Ts - (reply: 4)
  19. sequencing problems: chromatogram attached - messy peak data (reply: 6)
  20. Sequencing contamination? - (reply: 2)
  21. Expression Vector sequencing problem - (reply: 6)
  22. Expression Vector sequencing problem - (reply: 1)
  23. Primer extension and sequencing from total RNA - (reply: 1)
  24. Sequencing incorrect after digestion and ligation - (reply: 5)
  25. Sequencing of MSP products? - (reply: 5)
  26. cost of DNA sequencer? - (reply: 2)
  27. Sequencing pDNA - (reply: 3)
  28. PCR to sequencing problems - (reply: 5)
  29. is cDNA sequencing tricky? - (reply: 1)
  30. ِpeptide sequencing - (reply: 2)
  31. sequencing methods-when which one - (reply: 4)
  32. DNA sequencing - (reply: 1)
  33. sequencing problem - (reply: 3)
  34. TA cloning, sequencing results are vector self-ligation while bacterial PCR iden - (reply: 2)
  35. Sequencing result is vector self-ligation while bacterial PCR identification was - (reply: 5)
  36. DNA SEQUENCING - (reply: 1)
  37. Please help me analyze BSP cloned sequencing results. - (reply: 8)
  38. Bisulphite Sequencing of clones but getting several products - (reply: 3)
  39. problem of DNA sequencing - (reply: 3)
  40. Gel purification of large plasmids for PCR or sequencing, HELP! - (reply: 3)
  41. Sequencing:why do pcr AND cloning into plasmid? - (reply: 6)
  42. To whom working with DNA sequencing - (reply: 2)
  43. TOPO primer choice for sequencing - (reply: 3)
  44. sequencing vector DNA - (reply: 4)
  45. Cloning and Sequencing woes - (reply: 9)
  46. Bisulfite Sequencing: establishment - (reply: 1)
  47. advice re: shRNA vector sequencing - (reply: 4)
  48. Sequencing - (reply: 2)
  49. 16s or 23s for sequencing the unknown - (reply: 2)
  50. sequencing problem - (reply: 7)
  51. What is the cost of 454 sequencing? - (reply: 4)
  52. Degnerate PCR and Sequencing Reactions - (reply: 4)
  53. Bisulfite direct sequencing + cloning - (reply: 2)
  54. ESME software - Direct sequencing (reply: 1)
  55. difference in sequencing results - (reply: 8)
  56. sequencing not good after reading poly A tail..why? - (reply: 3)
  57. Bisulfite sequencing condition - (reply: 3)
  58. Bisulfite tx'd DNA sequence analysis - what to do with CG to TA conversion - bisulfite sequencing analysis (reply: 7)
  59. I need help sequencing a plasmid - pretty please, with sugar on top! (reply: 2)
  60. ligating into vector: sequencing trouble - vector sequences before RE but not after (reply: 1)
  61. problem with cloning and sequencing. - (reply: 10)
  62. Longest single DNA sequencing read? - (reply: 8)
  63. Sequencing protocol ....? - (reply: 2)
  64. Short and failed sequencing reads - (reply: 5)
  65. How to association the methylation profiling with the expression? - statistic questions about the bisulfite sequencing (reply: 1)
  66. Analysis of Quantititive Sequencing - (reply: 5)
  67. what's the difference between sequencing primer and cloning one. - confused (reply: 5)
  68. sequencing issue - Internal primers (reply: 9)
  69. direct bisulfite sequencing - (reply: 6)
  70. Checking clones by sequencing - (reply: 1)
  71. Free Minipreps and 50% off + Free Sequencing Clean-Up + Methylation Beta Kits - Free kit offers (reply: 3)
  72. N-terminal protein sequencing.. "holes" at lysine residues.. meaning? - what kinda modifications cud've happnd? (reply: 2)
  73. Need to get 3' end sequence! - 3' end sequencing (reply: 4)
  74. Sequencing slippage problem on ABI 3130? - (reply: 2)
  75. Bisulfite sequencing - why cloning? - (reply: 3)
  76. Problems sequencing DGGE products - (reply: 1)
  77. Overcoming PCR bias before bisulfite sequencing?! - (reply: 2)
  78. Different sequencing result of PCR products from the same cDNA - (reply: 2)
  79. wrong sequencing of T clones - (reply: 3)
  80. How to interprete direct sequencing results? - (reply: 26)
  81. Sequencing - (reply: 5)
  82. Trouble sequencing DNA from mutagenesis reaction - (reply: 1)
  83. Sequencing reaction failure- Only Background - Sequencing reaction (reply: 3)
  84. Nanog Methylation and sequencing - (reply: 3)
  85. Sequencing PCR products with biotin-labelled primer - (reply: 1)
  86. sequencing new "unknown" plasmid - any good SV40 primers out there? (reply: 1)
  87. Sequencing a 10kb length of dna in a 12kb plasmid - (reply: 3)
  88. Bisulfite sequencing of PCR products after AIMS technique - (reply: 3)
  89. sequencing result - sequencing result (reply: 2)
  90. DNA sequencing on the left and right chromosome - do we assume that left and right chromosomes have the same DNA sequenc (reply: 2)
  91. Sequencing problem :( - (reply: 1)
  92. cloning vs. direct sequencing - (reply: 1)
  93. sequencing - (reply: 3)
  94. What kit to use for bisulfite sequencing? - (reply: 1)
  95. Plasmid DNA Sequencing with Universal Primers - (reply: 3)
  96. MSCV LTRmir30 vector sequencing! - (reply: 2)
  97. BSP - which polymer do you use? - Which sequencing polymer gives the best results? (reply: 1)
  98. what more i do?(sequencing) - sequencing (reply: 11)
  99. BigDye® v1.1 & v3.1 5X Sequencing Buffer Makeup? - (reply: 1)
  100. Sample preparation for sequencing - (reply: 4)
  101. Controls for Bisulfite Sequencing - (reply: 13)
  102. Problem with shRNA sequencing! Help. - (reply: 3)
  103. strange sequencing / cloning problem with AvrII-site - (reply: 2)
  104. Bisulphite sequencing question - (reply: 8)
  105. Bisulfte PCR and sequencing PCR and Primer Notes - Tips and hints on PCR for bisulfite converted DNA and bisulfite primer (reply: 33)
  106. DNA sequencing company (cont.) - (reply: 3)
  107. Sequencing DNA - (reply: 2)
  108. DNA SEQUENCING PROTOCOL - (reply: 3)
  109. N-terminal sequencing - (reply: 1)
  110. Gene sequencing problem - Getting a non gene of interest (reply: 1)
  111. Miniprep for sequencing - (reply: 10)
  112. Sequencing from Minipreps - Is it possible to sequence directly from minipreps? (reply: 14)
  113. What do you mean by other letters aside from A, C, G, and T in DNA sequencing r - (reply: 3)
  114. pGEM v pTOPO PCR products for sequencing.... Help! - (reply: 3)
  115. Direct sequencing on BSP PCR products: problems with A and T stretches - (reply: 3)
  116. How good should automated sequencing be? - (reply: 1)
  117. Tips for sequencing miniprep DNA - (reply: 4)
  118. How to interpret sequencing results? - (reply: 3)
  119. miniprep DNA v PCR product, for sequencing - (reply: 4)
  120. use of thioglycolic acid in buffer - electrophoresis prior to N-terminal sequencing (reply: 2)
  121. How to determine the DNA sequence without sequencing? - (reply: 8)
  122. Noise in DNA sequencing peak reading - (reply: 2)
  123. DNA sequencer - (reply: 2)
  124. Restriction Enzyme and Sequencing Problems - (reply: 1)
  125. problem with sequencing - (reply: 3)
  126. DNA sequencing trouble - DNA sequencing (reply: 4)
  127. Sequencing SNPs basics - Can anyone explain? (reply: 4)
  128. wrong sequencing result?! - (reply: 13)
  129. Glycoprotein sequencing & PVDF membrane transfer - (reply: 3)
  130. Mutagenesis Help (plasmid isolation & sequencing stages) - (reply: 2)
  131. sequencing is alrite but still...... - (reply: 7)
  132. Weird sequencing result of my constructs - (reply: 4)
  133. A problem with bisulfite sequencing - (reply: 7)
  134. Bad sequencing result - (reply: 5)
  135. How to transfer the BSP sequencing results into the standard legend? - (reply: 8)
  136. Bisulfite Sequencing Technique - (reply: 8)
  137. sequencing cyclic peptides - (reply: 1)
  138. Sequencing of bisulfite treated DNA - (reply: 7)
  139. Concentrating proteins for N-terminal sequencing - (reply: 2)
  140. Video of DNA sequencing technique - (reply: 3)
  141. PCR amp. - sequencing problem - (reply: 3)
  142. sequencing cyclic peptides - (reply: 1)
  143. Problems in DNA sequencing after gel purification - (reply: 9)
  144. MSP and Bisulfite genomic sequencing results don't match! - (reply: 5)
  145. Sequencing - (reply: 5)
  146. rpoD sequencing - (reply: 2)
  147. Is the insert not complete? - Sequencing Cloned PCR product (reply: 5)
  148. Stick T7 on custom primer for sequencing? - (reply: 2)
  149. pwzl sequencing - (reply: 3)
  150. Sequencing Analysis - (reply: 7)
  151. Bisulfite sequencing primer - (reply: 4)
  152. References for direct sequencing - (reply: 1)
  153. Sequencing difficulties - (reply: 2)
  154. sequencing problem - (reply: 1)
  155. why do cloning before sequencing - (reply: 8)
  156. reading sequencing results - (reply: 4)
  157. Bisulfite sequencing results - how to analyze (reply: 1)
  158. cloning restriction fragment for sequencing - (reply: 1)
  159. plasmid sequencing - (reply: 4)
  160. sequencing through insertions sequences - (reply: 2)
  161. Sequencing problems-stupid question! - (reply: 5)
  162. sequencing problems - suggestions required (reply: 1)
  163. sequencing a restriction fragment - (reply: 4)
  164. How to design primer for sequencing unknown DNA - (reply: 5)
  165. Sequencing Primers - (reply: 4)
  166. Sod the sequencing - (reply: 10)
  167. Has anyone used this DNA sequencing product before? - (reply: 14)
  168. Direct bisulfite sequencing of PCR-products: degree of CpG methylation - (reply: 4)
  169. direct plasmid sequencing problems - direct plasmid sequencing problem (reply: 4)
  170. Sequencing difficulties - (reply: 7)
  171. pcr sequencing - (reply: 2)
  172. Problems with bisulfite sequencing - (reply: 11)
  173. DNA sequencing! I need a quick answer! - (reply: 2)
  174. No T's in sequencing print-out!? - (reply: 1)
  175. Sequencing primer dilution - (reply: 2)
  176. what is the best pair of sequencing primer for puc 18 - (reply: 1)
  177. pCI sequencing primer - (reply: 1)
  178. Which polymerase for bisulfite sequencing PCR - (reply: 3)
  179. designing the sequencing primer - (reply: 2)
  180. direct sequencing of bisulfite modified DNA - (reply: 5)
  181. bisulfite sequencing: cell lines vs tumors - (reply: 3)
  182. Best way to purify PCR product for sequencing - (reply: 2)
  183. Genomic sequencing - (reply: 34)
  184. antibiotics for 3 vectors and sequencing - (reply: 2)
  185. DNA sequencing kit - USB T7sequencing kit (reply: 3)
  186. sequencing with large plasmids - (reply: 4)
  187. dna sequencing amount - (reply: 4)
  188. Visualization of pcr product before sequencing - (reply: 5)
  189. topo cloning for sequencing? - (reply: 3)
  190. How To Make 2M Bisulfite Solution? - Bisulfite Sequencing (reply: 6)
  191. Inactivation of Thermo Sequenase / Taq polymerase - (reply: 1)
  192. Primer Programm for Sequencing - (reply: 3)
  193. DNA sequencing - (reply: 8)
  194. Detecting deletions via a sequencing trace? - (reply: 3)
  195. Bisulfite genomic sequencing - Bisulfite genomic sequencing (reply: 1)
  196. Sequencing plasmid gets overlapping peaks - troubleshoot (reply: 5)
  197. trouble with TOPO TA CLONING KIT for SEQUENCING - (reply: 8)
  198. Sequencing a RNAi-Construct! - A question! (reply: 2)
  199. Bisulphite sequencing -pick clones - (reply: 1)
  200. How to explain my MSP sequencing result? - (reply: 6)
  201. Pls check my bisulphite sequencing primers - (reply: 12)
  202. How to setup control for bisulfite sequencing PCR - (reply: 2)
  203. Bisulfite Sequencing Primer Design - (reply: 3)
  204. differents results in PCR and sequencing!! - (reply: 3)
  205. Reading chromatograms after bisulfite sequencing - Bisulfite sequencing (reply: 7)
  206. DNA sequencing problem - (reply: 9)
  207. Bisulfite sequencing -- Positive control? - bifsulfite sequencing (reply: 3)
  208. Bisulfite sequencing - (reply: 13)
  209. Sequencing of RT-pPCR products? - (reply: 1)
  210. Sequencing of PCR products - (reply: 5)
  211. distinguish between T and C after bisulfite sequencing - (reply: 3)
  212. Plasmid sequencing - (reply: 2)
  213. can I freeze the gel slice for protein sequencing - (reply: 1)
  214. Sequencing REPEATS - (reply: 4)
  215. Sequencing 40 kb long vibrio dna - Need some help on sequencing 40 kb long vibrio (reply: 2)
  216. Bisulfite sequencing problems - T>C conversion (reply: 13)
  217. 16s rRNA sequencing to determine bacteria present - (reply: 2)
  218. How to design primers for CpG, CpNpG, CpHpH methylation - Bisulfite Sequencing PCR (reply: 2)
  219. Bisulfite sequencing problems - when CpG sites are too much... (reply: 4)
  220. Sequencing of Plasmids - (reply: 2)
  221. Amplify long fragment from bisulfite treated template - for bisulfite sequencing (reply: 19)
  222. Sequencing Vendors - (reply: 2)
  223. Sequencing problems!! - homopolymeric region and slippage (reply: 8)
  224. No PCR Product for Sequencing - (reply: 5)
  225. DNA denaturing problem for bisulfite sequencing - (reply: 1)
  226. Sequencing bisulphite modified PCR- Very bad sequence?? - (reply: 1)
  227. Bisulfite sequencing PCR problem - no products (reply: 3)
  228. why don't do MSP product sequencing - (reply: 2)
  229. Need help in plasmid sequencing - (reply: 4)
  230. Cloning RACE products - Problem with sequencing ends (reply: 3)
  231. Bisulfite sequencing and PCR issues - (reply: 1)
  232. DNA quantification - How to quantify my PCR product before sequencing?? (reply: 8)
  233. Problems in bisulfite sequencing reproducibility - (reply: 7)
  234. Potentially dumb questions on methylation - Bisulphite sequencing queries (reply: 9)
  235. PCR product sequencing BD v3.1 - Help in removal of residues (reply: 1)
  236. Direct sequencing without cloning - how (reply: 3)
  237. Sequencing PCR products - (reply: 2)
  238. DNA sequencing problem - (reply: 1)
  239. Sample prep for sequencing - (reply: 2)
  240. Which in vitro transposition sequencing kit? - in vitro transposition (reply: 1)
  241. sequencing of Pcr product - (reply: 4)
  242. ABI377 Sequencing of PCR products - (reply: 1)