ِpeptide sequencing - (May/01/2008 )

Hi everyone:

1- I have identified the amino acid sequence of complement factor B using bioinformatics tools. my question is how can I confirm this sequence experimentally? Can any one help?

2- What type of experiment should I do to obtain the physical properties of CFB?

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q1: is it really an identification or only a prediction? you need the genuine nucleotide or amino acid sequence

q2: Mw (gel filtration), pI (chromato focussing, 2D GE), STOKE´s radius (sucrose density gradient centrifugation), sedimentation coefficient (sucrose density gradient centrifugation), friction ratio (sucrose density gradient centrifugation)

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1) If you can purify it, either Edman sequencing or you could try MS/MS
2) Also try CD for general structural data

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