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Nucleus Related Discussions
  1. What do cells look like after nuclear extraction swell/break step? - (reply: 4)
  2. Signal in the citoplasm instead of in the nucleus - (reply: 1)
  3. Marker for Sample from Nuclear Extraction - (reply: 2)
  4. Problems with nucleus isolation and 293 cells - Nucleus seems to disrupt. What to do? (reply: 1)
  5. Autoclaving nuclei isolation buffer - (reply: 8)
  6. Best method to get Cytoplasm extract? - without mitochondria or nuclear extracts (reply: 8)
  7. nuclear export signal finder? - (reply: 1)
  8. nuclear staining without permeablization - (reply: 3)
  9. Nuclear fraction marker for Western Blots - (reply: 4)
  10. Nuclear extract loading control in Western blot - (reply: 3)
  11. Which control protein for Western Blotting of nuclear protein? - (reply: 9)
  12. Removing histone proteins from nuclear protein isolation - (reply: 2)
  13. Propidium iodide staining of nucleus for IFA - troubleshooting required (reply: 6)
  14. Nuclear extracts from myeloid cells? - (reply: 9)
  15. Too low concentration of nuclear extract protein - after isolation (reply: 1)
  16. prediction of nuclear localization signal - (reply: 1)
  17. Nuclear Extraction kit for Primary Cell? - What's a good one? (reply: 1)
  18. What's a good Nuclear extraction kit for primary cell? - (reply: 1)
  19. Gentle extraction of nuclear protein complex - (reply: 2)
  20. Immunofluorescence for nuclear antigen - Immunofluorescence (reply: 3)
  21. Nuclear extraction of brain tissue for ChIP - (reply: 3)
  22. Suggestion for finding nuclear and nucleolar localization signals... - Online Softwares for localization signals... (reply: 2)
  23. Isolation of nuclear and cytoplasmic fractions - (reply: 3)
  24. Nuclei isolation for Chromatin IP - (reply: 5)
  25. Dissappearing nuclei - (reply: 2)
  26. cell fractionation and obtaining pure nuclei - (reply: 1)
  27. Intense cytoplasmic staining for Hoechst 33258? - not nuclei staining. (reply: 2)
  28. GFP, both in the nucleus and in the cytoplasm - transfection (reply: 8)
  29. nuclei staining and immunolocalization - (reply: 3)
  30. cytosolic and nucleus fractionation protocol plz - (reply: 2)
  31. Live cell nuclei immunostaining - (reply: 2)
  32. nuclear counter staining - TOPRO-3 (reply: 2)
  33. please help: how to isolate nuclear protein from tissue samples - (reply: 1)
  34. Nuclear Lysates Protein Quantification - Nuclear Extracts (reply: 4)
  35. Nuclear staining for 405 nm - (reply: 4)
  36. Issue with separating cytoplasmic and nuclear extract without contamination - (reply: 1)
  37. western blot for nuclear and cytoplasm seperation sample - (reply: 3)
  38. EMSA total or nuclear extract - (reply: 1)
  39. loading control for nuclear protein in rabbit cell extract - (reply: 1)
  40. Cell fractionation: nuclei do not pellet - (reply: 2)
  41. what nuclei look like after high salt nuclear extraction - (reply: 11)
  42. how to avoid cytosolic proteins in nucleus - (reply: 3)
  43. How can I separate nuclei from cytoplasm in trophoblastic cells - (reply: 1)
  44. NFkB nuclear staining problems - (reply: 3)
  45. Nuclear extract without DTT? - (reply: 3)
  46. subcellular fractionation confirmed by western not consistent with microscopy - nuclear protein by microscopy not nuclear in fractionated sample -WHY? (reply: 3)
  47. western with nuclear extract - does high salt/glycerol affect running (reply: 2)
  48. nuclear stain for living cell. any recommendation for dye? - (reply: 6)
  49. nuclei extraction of yeast (S.Cerevisiae) - any protocol? (reply: 1)
  50. immunostaining for PML nuclear bodies - Please enlighten me... (reply: 1)
  51. Immunostaining of cytoplasmic proteins in nucleus, why? - (reply: 10)
  52. Nuclear staining - How to permeabilise the nuclear envelope? (reply: 9)
  53. precipitation of nuclear proteins during GST pull down assay - (reply: 1)
  54. Advantages to eukaryotic cells of having a nucleus? - (reply: 9)
  55. normalization for nuclear extraction - (reply: 1)
  56. Tobacco nuclei extraction from plant tissue - Nuclei extraction (reply: 2)
  57. How to lyse plant nuclei? and keep nuclear protein complex stable - (reply: 1)
  58. NF-kB EMSA - Different nuclear protein extraction method leads to opposite results (reply: 1)
  59. NF-kB protein nuclear and cytosol isolation - No band separation in cytosol and nucleus (reply: 7)
  60. Nuclear staining instead of cytoskeleton - (reply: 12)
  61. nuclei fractionation for western blotting - (reply: 1)
  62. how to extract nuclear protein - help (reply: 8)
  63. Nuclear Marker for Western Blotting - (reply: 3)
  64. Help! Nuclear extraction! - (reply: 3)
  65. Nuclei isolation from mouse brain tissue - Nuclei isolation (reply: 1)
  66. nuclear import inhibitor - (reply: 1)
  67. RNA extraction - nuclear and cytoplasmic fractions - (reply: 3)
  68. immunoprecipitation of nuclear protein - (reply: 2)
  69. method of making nuclear extract for protein interaction? - (reply: 1)
  70. RT-PCR mouse RNA released from complement-lysed cells - Goal: to whether RNA is of nuclear or mitochondrial origin. Planning to PCR ampl (reply: 2)
  71. Can I extract nuclear proteins in transfected cells? - (reply: 2)
  72. Co-IP, nuclear proteins - buffers for lysis? - Who else does co-ip on nuclear proteins? (reply: 1)
  73. Nuclear staining - staining with a non-nuclear antibody - (reply: 1)
  74. Nuclear Extraction Protocol for Neurons - (reply: 2)
  75. Protocol for nuclei extraction or isolation using IGEPAL - (reply: 15)
  76. Separating bacteria from nuclei - (reply: 1)
  77. Nuclear protein in a post nuclear supernatant? - (reply: 6)
  78. isolating nuclei - how to address sticky nuclei (reply: 3)
  79. Plant nuclei isolation - (reply: 2)
  80. Nuclear extract - Protocol for nuclear extract for WB (reply: 2)
  81. Triton not permeabilizing nuclear envelope - (reply: 4)
  82. What is the best nuclear protein marker? - Fractionation (reply: 3)
  83. nuclear extracts - (reply: 2)
  84. Nuclear and cytoplamic markers - (reply: 3)
  85. Nuclear translocation - nuclear transport of proteins without NLS (reply: 1)
  86. Nuclear extracts for ChIP? - (reply: 1)
  87. nuclear extract - protocol for cell fractioning (reply: 1)
  88. where can I find protocols for nucleus staining? - (reply: 1)
  89. Is actin an internal control for nuclear fraction? - (reply: 3)
  90. How many cells (nuclei) per ml pellet? - (reply: 3)
  91. Fixation for IF??? - best method for nuclear antigens (reply: 1)
  92. Mitochondrial, cytosolic and Nuclear Protein extractions - An all in one protocol? (reply: 1)
  93. Nuclear Extract problem - (reply: 3)
  94. PROBLEM: Western blot of a 220 kd nuclear protein - (reply: 3)
  95. separation of nucleus protein - lysis buffer (reply: 1)
  96. CHIP with nuclei - (reply: 1)
  97. Nuclear protein extract (Scrape or Trypsinize)? - (reply: 1)
  98. isolation of nuclear protein - isolation of a nuclear protein, namely Akt protein (reply: 1)
  99. triton x and np40 for nuclei isolation - (reply: 3)
  100. The best way to get nuclear proteins from mammalian cells - How to break your cells? (reply: 1)
  101. Nuclear RNA knockdown with siRNA - is it possible? (reply: 6)
  102. How to transfect siRNA into the nucleus for transcriptional silencing? - Which transfection reagent? (reply: 9)
  103. nuclear localization signal - NLS (reply: 1)
  104. Purification of nuclear proteins - (reply: 2)
  105. can RIPA buffer use to prepare nuclear protein? - (reply: 1)
  106. nuclear extract - contamination cytoplasmic/nuclear extrac (reply: 8)
  107. Nuclear Extraction - (reply: 1)
  108. Isolation of nuclei from mammalian cells - (reply: 1)
  109. nuclear membranepermeabilization - (reply: 3)
  110. Nuclear receptors:Immunohistochemistry - (reply: 1)
  111. NUCLEAR EXTRACT - (reply: 1)
  112. Sigma nuclear protein extraction - (reply: 1)
  113. commercial nuclear extracts - (reply: 1)
  114. Isolation of mammalian nuclei for nuclear run-off assay - (reply: 1)
  115. nuclear extraction - internal control (reply: 2)
  116. Sigma Nuclear Protein ExtractionKit - (reply: 1)
  117. quantitation of nuclear extracts - (reply: 1)