Nuclear and cytoplamic markers - (Nov/16/2005 )

Hi everyone,

I would like to know what proteins would be good nuclear and cytoplasmic marker? I am extracting nuclear and cytoplasmic fractions and I want to make sure there is not contaminations.

Any suggestions would be good!

Thanks

Winnie

-Smap-

QUOTE (Smap @ Nov 16 2005, 11:57 PM)

Histone H1 will be a good marker for nuclear fraction. For cytoplasmic fraction GAPDH will work well

-Soni-

QUOTE (Smap @ Nov 16 2005, 11:57 PM)

or: tubulin for cytoplasmic, myc for nuclear..

-Jusu-

I have been using RCC1 (stressgen) as a nuclear marker, it works very well. As for the ctoplasmic marker I have been using gamma-tubulin, but there seems to be some in my nuclear fraction, esp when I fractionate using the Pierce kit. Does anyone have any suggestions about other cytoplasmic markers (I'll try GAPDH), but are other tubulins better? Also, what protocols are people using for fractionation?

thanks,
Seema

-Lakdawala-