Mitochondrial, cytosolic and Nuclear Protein extractions - An all in one protocol? (Jul/07/2005 )

Does anyone know of, or reference me to, a protocol that allows you to isolate mitochondrial, cytosolic AND nuclear proteins? Any protocols I've come across usually allow you to isolate either mito/cyto or cyto/nuclear but not all three. Can anyone help? Would be hugely grateful!!! Thanks!

You can do a subcellular fractionation by differential centrifugation...you know, separate the p1, hmf, lmf, and cf...