Dissappearing nuclei - (Feb/08/2008 )
After fixing my cells (tenocytes grown in monolayer) in ethanol and staining with methylene blue a lot of them have lost their nuclei. Has anybody any idea why this might happen? It's never happened before - is it related to senescence? 
-Dr Grumpy-
QUOTE (Dr Grumpy @ Feb 8 2008, 04:25 PM)
After fixing my cells (tenocytes grown in monolayer) in ethanol and staining with methylene blue a lot of them have lost their nuclei. Has anybody any idea why this might happen? It's never happened before - is it related to senescence?
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-Dr Grumpy-
Interesting phenomenon!
Maybe try fixing your cells in a different manner.
Maybe Methanol/acetic acid.
Does your protocol specifically call for etoh fixation?
-mikew-