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MicroRNA Related Discussions
  1. discuss about northern for miRNAs - northern, miRNA (reply: 3)
  2. Naming of miRNA - (reply: 2)
  3. What are normalisation primers for the rt-PCR? - Im going to order the mirVana rt-PCR kit for miRNAs (reply: 1)
  4. miRNA expression vector - (reply: 3)
  5. microRNA in situ hybridization - label probe 5' or 3'? (reply: 1)
  6. Validation of MicroRNA array results - (reply: 1)
  7. definition of miRNA family - (reply: 1)
  8. Exiqon miRNA arrays- any experiences - difficulties in validation (reply: 4)
  9. mirVana™ qRT-PCR miRNA Detection versus miScript SYBR Green PCR kit - advantages and disadvantages (reply: 1)
  10. Why do miRNAs always bind to 3'UTR? - (reply: 3)
  11. Ethanol or isoprapanol to precipitate miRNA? - (reply: 1)
  12. Spacing of artificial miRNA target sites for efficient knockdown - (reply: 1)
  13. miRNA 3' end nucleotidylation - (reply: 4)
  14. gene dowregulation by miRNA - (reply: 1)
  15. How to construct miRNA expression vector - (reply: 1)
  16. Discovery of novel miRNAs - (reply: 1)
  17. miRNA profiling - (reply: 6)
  18. miRNA introns and exons - (reply: 1)
  19. analyis of microRNA array data - (reply: 4)
  20. poorly conserved miRNA targets - conserved vs pooly conserved targets (reply: 1)
  21. miRNA and MRE difference - (reply: 6)
  22. miRNA poly A tailing - (reply: 2)
  23. miRNA family - (reply: 2)
  24. miRNA seed - understanding 5'UTR and 3'UTR (reply: 7)
  25. miRNA identification - miRNA prediction (reply: 2)
  26. promoter analysis of miRNA's - how to look for regulatory elements in the promoters (reply: 6)
  27. miRNA transfection concentraion issue - Question about transfection reagent ratio (reply: 1)
  28. miRNA microarray - mature and minor miRNA forms - (reply: 1)
  29. miRNA inhibition in vivo? - (reply: 1)
  30. totalRNA isolation w/ Trizol for miRNA Array - (reply: 3)
  31. siRNA vs. miRNA - (reply: 5)
  32. miRNA isolation and detection - (reply: 5)
  33. Using IRES in microRNA expression vector - (reply: 4)
  34. miRNA real-time using Sybr green - (reply: 9)
  35. Isoloation of total RNA and miRNA from blood ? - (reply: 1)
  36. miRNA northern blot using biotin probe - (reply: 2)
  37. What mml, gga, ssc, ggo, ppy.....mean? - MiRNA (reply: 5)
  38. miRNA cincentration? HELP needed! - (reply: 5)
  39. How to find miRNAs for one designated protein? - miRNA (reply: 1)
  40. miRNA prediction - (reply: 3)
  41. What software for windows vista can predict miRNAs in some new species whole-gen - (reply: 2)
  42. How many ug total RNA needed in miRNA Northern blot - please help me (reply: 2)
  43. miRNA* - miRNA star forms - (reply: 4)
  44. Which Kit should I use for microRNA microarray? - microRNA Microarray (reply: 3)
  45. Stable miRNA transfection - (reply: 7)
  46. miRNA array data and QPCR data correlation - (reply: 6)
  47. miRNA - cloning strategies - (reply: 2)
  48. difference between pre-miRNA and mature miRNA on Northern blot - (reply: 1)
  49. need help on microRNA real-time PCR - (reply: 6)
  50. labeling miRNA - (reply: 9)
  51. Mirna and Fungi - Microrna´s exists in Fungi ? (reply: 3)
  52. cDNA stability after miRNA stem-loop RT - (reply: 2)
  53. miRNA stability - (reply: 1)
  54. microRNA and FFPE - (reply: 1)
  55. is microRNA degraded with target? - is there any known pathway for miR degradation? (reply: 2)
  56. Detection of LNA miRNA knock-down by qPCR? - (reply: 5)
  57. Concentration of miRNA in cells? - (reply: 1)
  58. how to clone the genomic region for coding the mature miRNA - (reply: 2)
  59. PGL3, miRNA inhibitor co-transfection question - (reply: 5)
  60. miRNA detection problem by Northern blot - (reply: 4)
  61. miRNA target predicition - Too many targets come out... (reply: 6)
  62. How to find miRNA precursor (or gene) for a specific miRNA - (reply: 2)
  63. miRNA in situ hybridization - (reply: 5)
  64. miRNA & mRNA microarray data correlation - is there any software that can correlate miRNA and mRNA data? (reply: 4)
  65. 2'-O-methyl oligoribonucleotides as miRNA inhibitor - how many nucleotides in the oligo need to be modified (reply: 1)
  66. miRNA 3' end labeling - (reply: 4)
  67. Over-expresssion of miRNAs? - (reply: 5)
  68. (newbie) what makes siRNA and miRNA different - (reply: 2)
  69. miRNA lentiviral vector library - (reply: 1)
  70. miRNA gene knockdown? - (reply: 1)
  71. How can clone the complete miRNA primary transcript ? - (reply: 2)
  72. miRNA real time PCR - miRNA real time PCR (reply: 2)
  73. please recommend some common miRNA expression vectors - (reply: 2)
  74. RNA concentration issue for miRNA array - (reply: 4)
  75. Confusion about miRNA list - (reply: 4)
  76. knocking down mature miRNA with siRNA - is it possible to knockdown miRNA with siRNA? (reply: 4)
  77. miRNA in different size - (reply: 2)
  78. qRT-PCR and Northern blot - Is miRNA Northern blot necessary? (reply: 2)
  79. Morpholinos knockdown miRNA in Xenopus embryos - New paper (reply: 2)
  80. Can RNase A degrade miRNA? - (reply: 12)
  81. how to analyze transfection efficiency of miRNAs? - (reply: 3)
  82. Paper on miRNA knockdowns - Using Morpholinos for knockdown & specificity controls (reply: 1)
  83. miRNA luciferase reporter assay queries - (reply: 1)
  84. miRNA expression vector - miRNA expression vector (reply: 4)
  85. 3' terminal post-transcriptional processing of miRNAs? - (reply: 2)
  86. Trouble cloning miRNAs - (reply: 5)
  87. polyA tail as 3' adaptor to clone miRNA - (reply: 1)
  88. Has any one successfully performed miRNA cloning? - miRNA cloning troubleshooting (reply: 5)
  89. Uridines in linker design for miRNA cloning. - Why uridine is often used in the 3´-Linkers. (reply: 2)
  90. DNA contamination in microRNA sample - How to remove the genomic DNA from microRNA sample? (reply: 5)
  91. miRNA effect on 3'UTR cloned into a reporter vector - (reply: 2)
  92. new miRNA identification - looking for tool for identification of new miRNAs (reply: 2)
  93. non-radioactive miRNA probes - (reply: 2)
  94. chimeric RNA-DNA oligo probes for northern - sensitivity in miRNA northern detection (reply: 1)
  95. What is the best method to measure miRNA levels? - (reply: 2)
  96. Dicer knockdown to show miRNA mediated target mRNA expression? - (reply: 6)
  97. Total RNA extraction using TRizol for isolating miRNA - (reply: 6)
  98. Labeling of miRNA probes - (reply: 2)
  99. miRNA QPCR advice - taqman (reply: 3)
  100. overexpression of a microRNA cluster - (reply: 3)
  101. Taqman miRNA Assay Controls - not the usal question - (reply: 5)
  102. siRNA vs miRNA - why multiple terms for literally the same process? - (reply: 3)
  103. miRNA ladder problem - (reply: 5)
  104. Trouble with cloning specific miRNA and mRNA 3' termini? - (reply: 3)
  105. 2D protein gel for miRNA target screening? - (reply: 2)
  106. oligos as synthetic "miRNA" for quantification? - would an oligo / primer work in AB RT kit? (reply: 2)
  107. pri-miRNA transcript - get the exact primary transcripts of miRNAs (reply: 4)
  108. miRNA single or double stranded? - (reply: 11)
  109. how to know/predict a specific gene is regulated by miRNA - (reply: 7)
  110. miRNA northern protocol - (reply: 3)
  111. miRNA targets in comparison to siRNA targets - (reply: 2)
  112. Are miRNAs phosphorylated at the 3' end? - Labelling of miRNAs in a T4 RNA ligase reaction (reply: 3)
  113. miRNA extraction from primary tumor samples - (reply: 2)
  114. miRNA promoter ? - how to discover MicroRNA promoter element (reply: 1)
  115. miRNA quantification - easiest/cheapest method of measuring expression of a single miRNA in differnt ce (reply: 3)
  116. miRNA inhibitors - 2'-O-methyl versus Ambion antisense oligos (reply: 4)
  117. Express miRNA in vector for gene knockdown - (reply: 4)
  118. design artificial miRNA target - (reply: 3)
  119. How to overexpress miRNA? - (reply: 6)
  120. Knocking down miRNA using siRNA - (reply: 5)
  121. Two simple questions about microRNA's name - (reply: 2)
  122. miRNA for knockdown - RNAi (reply: 2)
  123. Has anyone tried miRNA isolation using the flash PAGE fractionator from Ambion? - (reply: 3)
  124. Exiqon (miRCURY) microRNA microarray - (reply: 3)
  125. Can't repeat a perfect miRNA real-time standard curve - (reply: 2)
  126. miRNA target prediction tools - (reply: 2)
  127. Control miRNA in experiments? - let-7 or miR-30??? (reply: 2)
  128. Stem loop RT-PCR for miRNA quantification - how can I design the stem loop primer? (reply: 2)
  129. overexpression of miRNA in a shRNA-vector? - (reply: 1)
  130. miRNA extraction using Trizol - (reply: 10)
  131. miRNA target claasification - (reply: 1)
  132. Puzzle about methylation on plant microRNA - (reply: 1)
  133. miRNA overexpression - pol II - type vectors... (reply: 1)
  134. how to get poly(A) tailed microRNA - (reply: 1)
  135. Has anybody tried miRNA Arrays from Ambion? - (reply: 9)
  136. Reverse transcription of poly-A tailed miRNAs using anchored Oligo dT primers?&# - (reply: 6)
  137. In Situ Hibridization of Very Small Sequences - Northern Blot of miRNAs (reply: 2)
  138. micro RNA traget prediction - (reply: 5)
  139. what is the difference about siRAN, miRNA, shRNA and RNAi - (reply: 1)
  140. microRNA Cloning - Advice...anyone...please! (reply: 5)
  141. BLAST EST to identify possible microRNA? - (reply: 3)
  142. miRNA databases - (reply: 8)
  143. mirVana miRNA detection kit - help (reply: 4)
  144. Identifying microRNA computationally? - (reply: 3)
  145. Conservation of miRNA's target site - (reply: 1)
  146. When is an miRNA not an miRNA? - definition of energy state (reply: 1)
  147. extraction of endogeneous mirna and sirna - (reply: 6)
  148. Detection of microRNA - (reply: 5)
  149. micro-RNA prediction algorithm - MiRscan or any other code for miRNA prediction (reply: 3)
  150. mirna alignment II - Alternatives to miranda? - looking for mutliple mirna alignment tools (reply: 1)
  151. microRNA alignment - (reply: 5)
  152. miRNA products - what would you like to see available? (reply: 2)
  153. What is the different between microRNA and siRNA? - (reply: 4)