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new miRNA identification - looking for tool for identification of new miRNAs (Apr/24/2007 )

Hi all,

I would like to identify new potential miRNA, does anyone know how to do this? Is there any software tool for this? I have found several softwares for identification of miRNA targets, but no for new miRNA... unsure.gif
Or are all potential miRNAs already found and published...
Thanks

-genetik-

QUOTE (genetik @ Apr 24 2007, 11:16 PM)
Hi all,

I would like to identify new potential miRNA, does anyone know how to do this? Is there any software tool for this? I have found several softwares for identification of miRNA targets, but no for new miRNA... unsure.gif
Or are all potential miRNAs already found and published...
Thanks


Why not to try some software in microRNA prediction? such as miRScan...
In the latest database, there are totally nearly 400 human microRNAs, but I've read a paper, and it said that the actual number of miRNA may be much more bigger than this. Especially for those expressed specifically in certain tissues or cell lines...

-MicroRex-

QUOTE (MicroRex @ May 2 2007, 06:33 PM)
QUOTE (genetik @ Apr 24 2007, 11:16 PM)
Hi all,

I would like to identify new potential miRNA, does anyone know how to do this? Is there any software tool for this? I have found several softwares for identification of miRNA targets, but no for new miRNA... unsure.gif
Or are all potential miRNAs already found and published...
Thanks


Why not to try some software in microRNA prediction? such as miRScan...
In the latest database, there are totally nearly 400 human microRNAs, but I've read a paper, and it said that the actual number of miRNA may be much more bigger than this. Especially for those expressed specifically in certain tissues or cell lines...



Thank for your replay. I tried the miRScan, but I could not get it to work.. dry.gif
I used the example sequence - but the sever is kind of not responding. I inserted the sequence in this format:
mir-34 GCCAG....GCCC CGGC....GCCC
Is this correct?

-genetik-