How many ug total RNA needed in miRNA Northern blot - please help me (May/31/2008 )
hi, everyone. I am a newcomer, I will do miRNA northern blot recently, but I can not know how many total RNA will be needed.
-forrest gump-
QUOTE (forrest gump @ May 31 2008, 08:12 AM)
hi, everyone. I am a newcomer, I will do miRNA northern blot recently, but I can not know how many total RNA will be needed.
In these links, you may find answer for this.
http://search.vadlo.com/b/q?keys=miRNA&...mp;sn=158621799
hth/
-cellcounter-
QUOTE (forrest gump @ May 31 2008, 08:12 AM)
hi, everyone. I am a newcomer, I will do miRNA northern blot recently, but I can not know how many total RNA will be needed.
I believe that 20-40 ug Its more than enought.
-msimoes-