miRNA and MRE difference - (Aug/27/2008 )
Hi,
I'm new to miRNA. What's the difference between the seed region and MREs (miRNA Regulatory Elements)?
THanks
MRE is microRNA recognition element, which is normally at the 3' UTR of the target mRNA.
...so the seed region (on the miRNA) is at least nearly complementary to the MRE (on the mRNA).
Thank you.
So, the MRE on the mRNA (3'UTR) recognises the seed region on the miRNA (5'UTR).
There is one paper which cloned the MRE's of a few genes in a reporter assay and also then followed the experiemnt by cloning teh 3'UTR's of the same genes in reporter assay. WHy not just study the 3'UTRs directly instead of having to study the MRE's first?
paper: Lu Z, Liu M, Stribinskis V, Klinge CM, Ramos KS, Colburn NH, Li Y.
MicroRNA-21 promotes cell transformation by targeting the programmed cell death 4 gene.
Oncogene. 2008 Jul 17;27(31):4373-9. Epub 2008 Mar 31.
So, the MRE on the mRNA (3'UTR) recognises the seed region on the miRNA (5'UTR).
There is one paper which cloned the MRE's of a few genes in a reporter assay and also then followed the experiemnt by cloning teh 3'UTR's of the same genes in reporter assay. WHy not just study the 3'UTRs directly instead of having to study the MRE's first?
paper: Lu Z, Liu M, Stribinskis V, Klinge CM, Ramos KS, Colburn NH, Li Y.
MicroRNA-21 promotes cell transformation by targeting the programmed cell death 4 gene.
Oncogene. 2008 Jul 17;27(31):4373-9. Epub 2008 Mar 31.
Quite the opposite, in reality, the seed region recognises the MRE on mRNA 3' UTR.
If you study only the whole 3'UTR you can't be certain that the effect of your miRNA on a specific MRE, thus you have to clone the MRE and confirm your result. another good thing to do would be a construct bearing one or more mutations into the MRE, that should do it.
So, the MRE on the mRNA (3'UTR) recognises the seed region on the miRNA (5'UTR).
There is one paper which cloned the MRE's of a few genes in a reporter assay and also then followed the experiemnt by cloning teh 3'UTR's of the same genes in reporter assay. WHy not just study the 3'UTRs directly instead of having to study the MRE's first?
paper: Lu Z, Liu M, Stribinskis V, Klinge CM, Ramos KS, Colburn NH, Li Y.
MicroRNA-21 promotes cell transformation by targeting the programmed cell death 4 gene.
Oncogene. 2008 Jul 17;27(31):4373-9. Epub 2008 Mar 31.
Quite the opposite, in reality, the seed region recognises the MRE on mRNA 3' UTR.
If you study only the whole 3'UTR you can't be certain that the effect of your miRNA on a specific MRE, thus you have to clone the MRE and confirm your result. another good thing to do would be a construct bearing one or more mutations into the MRE, that should do it.
Thank you so much for the clarifications. Can you suggest a good read for miRNA for beginners? I'e been reading reviews by Pillai.
Thank you so much for the clarifications. Can you suggest a good read for miRNA for beginners? I'e been reading reviews by Pillai.
See attachments.
the first one is a new review, probably this is what you need to start. this one is quite "general", an overview on miRNAs but is new and up to date.
the second one is "specific", but there is a good part on luciferase assay (i know it because i'm the 2nd author....
).All the rest is up to you, there are MANY useful publications on many fields, so suit yourself.
Hope it helps
Fizban