miRNA target prediction tools - (May/11/2006 )

Hi¡¡ I have several doubts:

1.- How can I get a windows version of miRanda?

2.- Is it the best program for target prediction?

3.- What do you opine about other softwares like microInspector?

4.- When I upload my UTR sequence, I obtein many hits, most of them with a score >150. However, when I analyze these hits, I see there isn´t perfet matching in the seven nucleotides more 5´. Do you think it might be worthwhile to try validate these results?


lots of thanks

Sorry for my English

1.- How can I get a windows version of miRanda?
You can get the windows version here http://www.microrna.org/src/jmiranda/

2.- Is it the best program for target prediction?
I like miRanda.

3.- What do you opine about other softwares like microInspector?
Never use it.

4.- When I upload my UTR sequence, I obtein many hits, most of them with a score >150. However, when I analyze these hits, I see there isn´t perfet matching in the seven nucleotides more 5´. Do you think it might be worthwhile to try validate these results?
It is very hard to say. Even you have gotten a perfect match, you are not 100% sure that it is a miRNA target, let alone partial matches. I have not seen much vigorous evidence showing that mere seed sequence match is a true target.

OK. Thanks a lot for your help