making aliquote - i am confused it will affect my result (May/10/2008 )

after a long time i am writing here...hope everybody is fine........
now a days i am doing a lot of qRT-PCR...........i am tired of making aliquote of samples.........

My question is ..can i make the aliquote for standards or negative control or my cDNA sample in a large vol and then preserve it in 4 (or -20???) centigrade and use when i need

suppose I use for 1 sample
10 ul SYBR green
2 ul cDNA sample and
6 ul water

so total 18 ul and can i make a aliquote of 100 sample that mean a mixture of 1800 ul and then preserve it for future use??

i am afraid it will affect my result..........
please suggests me........

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I don't see any problem with that, but aren't you using commercial 2X master-mixes containing SYBR green? That is highly advisable.

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thank you
i use master mix containing sybr green from Roche

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Do you plan to make a huge pre-mix with primers and everything except template cDNA or you want to aliquote a template with sybr and only add different primers (it's not clear from your example)?
The first one is OK, but the second one is not a very good idea. To be comparable, all the samples should be from one pre-mix, not different ones.

Also as regents in Roche SYBR mix are stable on 4 degs for a month, primers not that much and cDNA definitelly not that much (though Roche claims the machine-ready reaction mix to be stable for 24 hours on RT, and I once had one plate in fridge for a week and it went well), and on the other hand the SYBR mix shouldn't be refreezed repeatedly. But if you plan to have a mix with sybr, primers and water for a reasonable time, like week or two, fridge shouldn't be a problem.

To reduce pipetting you can try mixing the forward and reverse primer to one vial, that's one pipetting step less.

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