RNA extraction from leukocytes - (Aug/06/2007 )

Hi all

I need to do RNA extraction from the human blood leukocytes. I get 1-5 mL of blood samples and will be needing to use the plasma for other studies.

I have heard a lot abt PAXgene blood RNA kit but I wonder if I could use it since I wold need to get plasma separated before I can extract RNA from the leukocytes. So far I have tried using RNeazy micro kit but have got very low yields of RNA from the samples.

Can anyone suggest me any better kit or method I could use to meet my objectives?

many thanks
Rits

-rits-

<ell i would go for trizol or quiazol or trireagent purification method.

-fred_33-

QUOTE (rits @ Aug 6 2007, 12:00 PM)

many thanks
Rits

Rits, I use the PAXgene kit for that purpose. You have to use the special tubes for your original blood collection instead of a serum or EDTA tube. You will get a much better yield of RNA, however the PAXgene tubes are VERY expensive.

If you decide to switch to this method, I would also suggest that you increase the centrifugation time for the original blood tubes.

I've used the RNeasy kit as well, but the yield is no where near the same.

Good Luck!

-AlyssaK-