Albumin Removal From BSA - (Jan/10/2013 )
Hi,
I have 250 Bovine serum samples. I would like to remove Albumin from these samples to have the chance to visualize the low abundant Immunoglobulin (IgA and IgM) by FT-IR.
If anyone know protocol to precipitate or remove albumin from these sample just write it to me.
Thanks,
Hema
-Hemavet-
You can use Affinity Chromatography or Ion Exchange Chromatography (Isoelectric Point). If you have less amount maybe with sepharose beads.
-Pangea-