Desalting resin - (May/17/2013 )

we have sephadex g-25, sephadex g-50 and sephadex g-75 resins. our protein is 20 Kda and we would like to desalt it for further processing. Can some one recommend which of these will be suitable. i.e which will cause less dilution and better desalting.

we would be packing them in XK 16/40 column and use for desalting so what amount of sample can be desalted at a time.

kindly suggest

thanks

see my reply in the "protein and proteomics" forum.

please don't post in multiple fora.