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Ligation Related Discussions
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Ligation problem again - (reply: 4)
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BamHI/XhoI ligation - (reply: 3)
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ligation problems....? - (reply: 1)
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self ligation - (reply: 5)
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Ligation buffer - DTT in ligation buffer (reply: 1)
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Help, no insert, ligation product couldn't be cut anymore! - (reply: 3)
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my strategy of ligation - (reply: 5)
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Blunt end ligation problem - (reply: 3)
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directional cloning problem - two-digested vector and insert - ligate - no colonies?? (reply: 2)
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Blunt end ligation problem - (reply: 2)
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Inverse PCR ligation - (reply: 1)
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ligation problem by using single RE - (reply: 8)
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blunt-end ligation - (reply: 2)
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T4 Ligase problem - Please help me, (reply: 10)
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ligation and transformation problems - (wrong) oligos (reply: 4)
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3 piece ligation - (reply: 4)
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Unique cohesive overhangs cloning + cohesive/blunt ends cloning - molar ratio / use of PEG in cohesive-blunt ligation (reply: 8)
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"ladder" ligation result - cloning is the bane of my existance (reply: 18)
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before electro-transformation, remove T4 Ligase or not? - (reply: 3)
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shRNA ligation problem - (reply: 3)
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pUC19 ligation - (reply: 1)
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Ligation problems... - (reply: 2)
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Why use 200 units of ligase? - I am going cuckoo lol (reply: 11)
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blunt ligation of large fragment - (reply: 6)
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ligase temperature - (reply: 5)
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transformation issues with ligation product - a double-digested (HindIII / HpaI) vector won't take up an insert (reply: 5)
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My first ever 3-way ligation :) - Advice on ratios? (reply: 5)
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Problem with Ligation Controls HELP PLEASE - EcoR1 cut site on pEGFPC2 vector will not religate? (reply: 8)
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question in ligation - (reply: 1)
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Ligation problem - 2 Questions (reply: 5)
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PEG 4000 for ligation - (reply: 5)
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3 way Ligation Issues... - (reply: 11)
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It keeps getting better and better lol my ligase might be out to get me - (reply: 2)
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Insert/vector ratio for ligation - (reply: 3)
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Ligation using TA cloning - (reply: 1)
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blunt end ligation - (reply: 2)
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ligation failed - (reply: 1)
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TA cloning and 3 fragment ligation problems - (reply: 1)
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Ligation problem - (reply: 7)
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Ligation SalI / XhoI - (reply: 8)
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Random DNA library problem - repair, ligation, digestion problem? TOPO and Zero Blunt (reply: 7)
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Ask about linker ligation - (reply: 6)
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how long can I keep a ligation reaction going at 4 C? - (reply: 3)
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methylation affects ligation? - (reply: 2)
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Sequencing incorrect after digestion and ligation - (reply: 5)
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Blunt end ligation - (reply: 8)
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DNA gel to Test Ligation? - (reply: 2)
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dilute the ligation reaction for electroporation - (reply: 7)
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Ligation failure - (reply: 1)
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pcr,ligation and transformation with RE SalI and XhoI - (reply: 4)
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double digestion and ligation - (reply: 3)
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3 piece ligation - (reply: 2)
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Check the ligation product, but got two fragments with strange sizes on the gel - (reply: 7)
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Ligation: time and temperature - (reply: 8)
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ligation - very small insert - problems during ligation (reply: 11)
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Poor ligation efficiency using Invitrogen TOP10 and XLI-blue cells - (reply: 5)
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Second-strand synthesis - why use ligase? - DNA polI - mediated SSS (reply: 1)
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About Ligation Independent Cloning - share the opinions (reply: 3)
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Ligation Reaction - (reply: 1)
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ligation problem - (reply: 4)
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Ligation problem? - (reply: 10)
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setting up an ligation reaction - (reply: 5)
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Problem with ligation..! - (reply: 5)
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questions about mung bean nuclease and ligation - (reply: 1)
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No ligation! Can't understand why! - (reply: 13)
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purification、ligation and transformation problems - (reply: 11)
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ligation failure-what to do? - tried everything but still not getting transformants (reply: 12)
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ligation transformation.......which chemically competent cells are the best? - (reply: 3)
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transfection with ligation mix? - (reply: 6)
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long insert ligation - (reply: 5)
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Big fragment ligation - (reply: 6)
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Small fragment sticky-end ligation into EGFP-N1 vector - (reply: 2)
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Do you discard phosphatase before ligation? - (reply: 6)
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T4 DNA ligase still usable after a night out? - (reply: 3)
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Ligation problem with PCR product cloning - (reply: 9)
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PCR cloning-primer design and ligation - (reply: 20)
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LIGATION DOESNT WORK - (reply: 7)
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Help! Suggestions for ligation ratios - (reply: 8)
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Ligation and Transformation Problem - (reply: 2)
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Klenow - ligation problem - Can Klenow add some extra nucleotides.. (reply: 7)
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Ligation, plasmid rescue problem! - PLasmid transformation after ligation problem (reply: 7)
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Tips on improving ligation+transformation from gel extraction - Let's contribute! (reply: 1)
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Question about ligation! - Ligation of 6kb insert in pGEM T-vector (reply: 1)
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Can i introduce strand DNA(not ligate with the plasmid) into E. coli? - (reply: 2)
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Need help for 3 fragment ligation using pGEM T - (reply: 7)
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Which enzyme for repairing nicks in ligated constructs? - (reply: 1)
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BLUNT END LIGATION - DIFFICULTIES ON BLUNT END LIGATIONS (reply: 3)
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Reaction volume for ligation - (reply: 5)
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Great ligation problem - Double digested DNA insertion problem (reply: 5)
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Blunt ended ligation not working! - (reply: 3)
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Absolutely no Ligation product - (reply: 6)
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pros and cons of Blunt vs sticky ligation - (reply: 2)
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Blunt end ligation problems, no ligation at all... - (reply: 2)
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4 enzyme digest and 3 piece ligation? - (reply: 3)
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ligation problem - (reply: 3)
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Self Circularization Problem with a 6KB vector - self ligation of a 6KB vector (reply: 1)
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cloning, ligation, dephosphorylation - ligation, dephosphorylation (reply: 3)
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Ligation of small insert - (reply: 4)
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unable to clone into pET29b and pET32b - sticky end ligation failing (reply: 2)
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How long do you ligate? - (reply: 6)
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Restriction enzyme sites mutate after ligation? - (reply: 2)
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Cloning without any digestion and ligation prosedures - GATEWAY Cloning Technology (reply: 3)
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Ligation problems using NEB Quick Ligase Kit - (reply: 5)
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DNA disappeared after ligation - (reply: 2)
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Need help with TOPO TA cloning problem! - TOPO TA clonig, ligation, no insert (reply: 23)
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Why Ligation and transformation not working? - (reply: 3)
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why the difference in ligation temperature? - (reply: 1)
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Ligation for two overnights. Is it bad? - (reply: 2)
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Ligate with 01 enzyme site - (reply: 5)
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Ligase always needed for DNA circularisation ? - (reply: 5)
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Transformation problem with newly ligated plasmids - (reply: 1)
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Product of ligation storage - (reply: 3)
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short oligos ligation and transformation - (reply: 3)
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multi fragment ligations - (reply: 2)
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T4 ligation problems with adapters - (reply: 1)
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getting faint band during ligation - (reply: 7)
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problem with ligation of big fragments - (reply: 3)
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ligation problem - (reply: 5)
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Unable to ligate two PCR products - (reply: 4)
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ligation - (reply: 5)
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how to ligate a gene repeatedly - (reply: 6)
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Is my DNA clean enough for a succesful ligation? - (reply: 15)
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Verifying Restriction sites generated in PCR of insert for subcloning - No colonies in transformation!Ligation issues?Insert issue? (reply: 4)
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Plasmid Ligation does not work-suggestions? - (reply: 6)
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ligation transformation kit - fermentas kit (reply: 4)
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Circular ligation product migration on gel? - (reply: 1)
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Ligation and transformation problem - (reply: 4)
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concentration of vector in ligation reaction - (reply: 4)
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Why the ligation does not work? - (reply: 7)
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Problems after gel purification of plasmids - Restrictases and ligase do not work on gel purified DNA (reply: 2)
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three way ligation - three way ligation help (reply: 6)
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Ligation with T4 DNA Ligase - (reply: 9)
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5' adaptor ligation fail - (reply: 3)
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How much ligase per ug of DNA? - (reply: 6)
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Dephosphorylation for one cohesive, one blunt end ligation? - (reply: 1)
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ligation step in cosmid library construction - (reply: 3)
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3 molar insert excess - how imp. is it for the ligation rxn (reply: 2)
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Quick T4 DNA ligase - optimizing the condition for quick ligase (reply: 6)
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ligation problem - (reply: 11)
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Ligation of purified PCR product to Topo plasmid (with T7 promoter) and Transfor - (reply: 2)
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T4 DNA ligase Buffer - (reply: 3)
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T4 DNA ligase - (reply: 1)
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Is time of ligation insert and vector crucial? - (reply: 4)
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Help with Ligation - (reply: 3)
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Ligation - (reply: 5)
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Problem with Ligation - (reply: 4)
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blunt ending the vector followed by the ligation - (reply: 1)
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Multiple fragment ligation - (reply: 7)
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HELP: Ligation Problems - (reply: 10)
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three-point ligation - (reply: 6)
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Can't ligate 2 linear fragments with cohesive ends - (reply: 5)
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Ligation of pBBR with PCR fragments doesn't work - (reply: 3)
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does the high pH of milli Q water will affect my ligation? - (reply: 8)
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T4 DNA ligase - (reply: 1)
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Double-stranded oligo ligation - (reply: 13)
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PCR and Digest cleanup before ligation. - A shortcut by avoiding gel purification. (reply: 15)
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problems in ligation/transformation - (reply: 12)
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ligation ratio - (reply: 1)
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Ligation with 3 inserts - (reply: 1)
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Can a single restricted vector spontaneously ligate back to a circular DNA? - (reply: 3)
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Quick ligation and blunting kits NEB - (reply: 7)
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Ligation Difficulty - (reply: 2)
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ligated vs. supercoiled - (reply: 8)
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Lots of colonies but they're all blue! - Do I need to adjust ligation ratios? (reply: 11)
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Cloning of synthetic oligos - how much oligos for ligation? (reply: 3)
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"Ligate" two overlapping pcr products with PCR fusion? - (reply: 1)
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Ligation/ transforamtion troubles - (reply: 2)
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digestion and ligation with BspH1 - digestion with BspH1 of insert , and Nco1 for the vector ,ligation pos (reply: 2)
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Problem with Digestion or Ligation. How to tell? - (reply: 7)
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Double digest question for ligation - (reply: 4)
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Ligation reaction gel picture - (reply: 1)
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Cloning/Ligation: How not to have Insert/insert binding? - (reply: 5)
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Cells grow on Amp plates but not in LB with Amp - Amp problem, bug strain problem, or ligation problem ? or all of the a (reply: 7)
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should I go on to the ligation? urgent - (reply: 4)
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blunt end ligation problem - (reply: 4)
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cloning/ligation problem? (shRNA into vector) - cloning, colonies in neg control (reply: 4)
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Ligation ratio problem - (reply: 2)
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transformation:more colonies with control! - control plate twice as many colonies as ligation (reply: 8)
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Ligation:blunt vs single base overhang - an old question? (reply: 5)
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Weird result (ligation) - (reply: 13)
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ligation and miniprep - (reply: 6)
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two fragment ligation - (reply: 3)
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How about 4 DNA fragments ligation and cloning - Cloning by 4 DNA fragments ligation and transformation (reply: 6)
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Ligation of half blunt PCR fragments - (reply: 4)
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Digestion, Ligation or transformation problem? - (reply: 8)
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preciptiate in Fermentas T4 ligase buffer - (reply: 1)
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about ligation problem - insert and vector size (reply: 9)
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Ligation at 16°C,ON: where to keep it till transformation? - (reply: 4)
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Ligation of a 1.8kb insert into 10.8kb vector - (reply: 4)
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Ligation into pET28 - (reply: 9)
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how to ligate random oligos to vector to construct a library? - (reply: 6)
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Is it posible to make ligation with DNA digested and stored at -20 - (reply: 2)
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Ligation insert without PCR? - (reply: 6)
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How many ligase units? - probably a very stupid question... (reply: 4)
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why does ligase buffer precipitate? - is it ok just to vortex it?? (reply: 12)
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How to solve the problem of toxic ligation? - Are there any other competent cells better than DH5a dealing with toxi (reply: 3)
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volume of ligation product - (reply: 6)
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Strange result after ligation and transformation - (reply: 2)
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klenowed PCR fragment into EcoRV digested bluescript - blunt end ligation proble - (reply: 5)
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very easy sticky end ligation: no colonies! - Cloning masters, please help doomed cloner! (reply: 28)
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Trouble with ligation - (reply: 7)
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How long for ligations? - (reply: 11)
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Question about phosphorylation and ligation - (reply: 1)
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ligation problem - (reply: 6)
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blunt end ligation after inverse PCR - (reply: 5)
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Ligation problems - (reply: 12)
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Multiple ligation - How many digestion products is it possible to ligate at the same time ? (reply: 6)
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Ligation failed? - (reply: 3)
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38bp insert and 6.7kb vector ligation problem - the ligation efficiency is too low (reply: 8)
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Are miRNAs phosphorylated at the 3' end? - Labelling of miRNAs in a T4 RNA ligase reaction (reply: 3)
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Agaroase gel analysis of ligation reaction - (reply: 6)
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Help with ligation using Topo TA - (reply: 7)
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Clonning problems - my ligation didn't work (reply: 4)
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transformation troubles - where to go next? - transformation/ligation (reply: 11)
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forgot to digest PCR insert before ligation. - what happened? (reply: 7)
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new puzzles on my 4-way ligation results - (reply: 6)
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ligation and proof reading Taq - (reply: 4)
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ligation product- direct pcr - (reply: 2)
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is amplification directly from my ligation reaction possible? - (reply: 1)
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problems in ligation of pET28b(+) - Ligation (reply: 5)
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genomic insert ligation - (reply: 3)
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one question on the 4-way ligation - (reply: 10)
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a large insert ligation - (reply: 2)
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ligation - (reply: 3)
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Fuzzy bands on agarose gel after ligation - (reply: 7)
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ligation ratio insert-vector - (reply: 6)
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ligation: only false positives. Advice needed - (reply: 2)
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can I take some of ligation mix and digest? - (reply: 4)
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Control PCR in Ligation - Genomic DNA + linker ligation (reply: 1)
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cloning experimental design to ligate three PCR products into one plasmid - (reply: 4)
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Multiple ligations - (reply: 3)
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Double Blunt Ended Ligation? - (reply: 8)
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T4 DNA ligase and RNA - (reply: 2)
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Ecoli genomic recombination causing cloning problems - after ligation vector size and sequence change (reply: 5)
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one blunt and one sticky ligation...something wrong - HELP!!! (reply: 2)
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Ligation problem, might be bc/ it's the 5kb insert? - Vector and insert are pretty much same size.. and it's not workin in a ' (reply: 3)
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ligation never works even for single cut vector!Why so!Help! - (reply: 7)
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Block-it Plasmid Ligation - (reply: 8)
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Multiway Ligations; sharing the art - Three way ligation and above (reply: 3)
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Troubleshooting Ligation/Transformation - (reply: 8)
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ligase inhibitors - (reply: 5)
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VECTOR LIGATION WITH One RE - (reply: 4)
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Afl II linearization & ligation - (reply: 3)
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using diluted ligase - (reply: 3)
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Should I purify digested vector before ligation? - (reply: 7)
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Ligation and cloning failure - (reply: 3)
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restriction direct to ligation? - skip clean up? (reply: 12)
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Linkers:insert ligation problem - (reply: 3)
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Ligation and Cloning Problem - Cloing Problem with pBSK (reply: 1)
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Freeze a complete ligation? - (reply: 3)
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help with ligation and gel extraction - (reply: 5)
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Problems with gel extraction and ligation - (reply: 12)
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Digestion or Ligation problems, Help! - Help!!!!!! (reply: 5)
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more ligation problems! - (reply: 4)
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Ligation of incompatible overhangs - (reply: 8)
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Ligation or transformation problem - (reply: 12)
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complete ligation HELP?! - (reply: 1)
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weird blunt-sticky ligation case - (reply: 2)
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Ligations with T4 DNA ligase - (reply: 4)
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restriction to ligation - (reply: 5)
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Wrong sized PCR band following digest & ligation into vector - :( please help... (reply: 1)
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Ligation Failure - 20 colonies tested with no results. Need advice (reply: 2)
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ligation only concatenate and not circularize - (reply: 2)
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Ligation failure using three DNA fragments. - Cohesive ligation failure (reply: 4)
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how to ligate two ends with different enzyme site - (reply: 3)
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Problems about ligation - (reply: 4)
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Unable to obtain colonies from PCR-insert + vector ligation - (reply: 6)
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ligation - (reply: 1)
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is there a restriction about amount of vector in a ligation? - (reply: 14)
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Triple ligation with IRES - (reply: 2)
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About Ligation :ph34r: - (reply: 1)
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blunt-end ligation problem - (reply: 3)
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Tips for transformation - which volume of ligation product? purification before transformation? Quantity o (reply: 4)
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Role of PEG in ligation - (reply: 3)
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Does the T4 ligase works perfectly well in the presence of TAE and EtBr? - (reply: 1)
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Blunt end ligation help - (reply: 2)
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What happens to my ligation system? - Very Very urgent! (reply: 3)
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No colony after ligation? - (reply: 3)
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TA ligation - (reply: 4)
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Ligation of multi-fragments? - Has anybody tried to ligate more than 02 fragments in a single experiment? (reply: 2)
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Ligation drama/trauma continues - did this work? - (reply: 2)
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Ligation over a six day period - WOuld this have damaged the DNA? (reply: 4)
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No colony after ligation - (reply: 6)
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Ligation worked - maybe? - Aimikins - I've got colonies! Which size do I pick? (reply: 4)
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toxic gene ligation/transformation URGENT HELP! - (reply: 1)
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how to move for ligation with low conentration of digested plasmid - (reply: 2)
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problem in ligation and transformation - (reply: 18)
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which quick ligation kit should I use ? - (reply: 1)
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Q for ligation ? - (reply: 1)
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Is it obligatory to precipitate DNA from ligation before transformed into E.coli - (reply: 7)
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ligation problem - (reply: 2)
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ligation question! - (reply: 2)
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No colonies in my ligation - (reply: 3)
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ligation trouble - (reply: 2)
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How to sequester SDS in order to do ligation? - (reply: 6)
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ligation problem - (reply: 1)
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phosphatase removal in ligation - (reply: 3)
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temp for ligation - (reply: 2)
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DNA purity for ligation - (reply: 1)
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I need help --- ligation troubleshooting - (reply: 9)
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ligation with sticky and blunt ends - (reply: 3)
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ligation inhibitors - (reply: 2)
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ligation of two DNA fragments - (reply: 1)
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Double-stranded RNA to RNA ligation - (reply: 1)
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ligation into pcDNA3.1(-) - (reply: 3)
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Is re-sequencing needed for insert ligated into a second vector? - (reply: 1)
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how to confirm ligation is successful - (reply: 3)
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Help on digestion and ligation...colonies not wanted why? - (reply: 4)
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problem with digestion and ligation of BgI II and kpnI RE - (reply: 1)
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Ligation and transformation - (reply: 3)
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gel purification necessary for ligation? - (reply: 3)
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purification of DNA extracted from agarose gel - does ethidium bromide remains / interefrence with ligation (reply: 1)
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Is it necessary to inactivate ligase after ligation? - (reply: 2)
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Ligation without insert shows colonies - (reply: 1)
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does glycogen inhibit enzyme digestion or T4 ligation? - (reply: 11)
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why my ligation can not work - (reply: 2)
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Ligation Problem - Molecular Biology (reply: 2)
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Ligation Problems - (reply: 5)
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blunting with no success! - blunt end ligation (reply: 1)
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can you ligate gel slices directly? - (reply: 8)
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gel purification of the vector... - is it good enough for ligation? (reply: 7)
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Ligation of big vs small - (reply: 3)
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KpnI Ligation Mystery - Bidirectional Cloning Working Unidirectionally (reply: 5)
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Do you kill the ligation product? - (reply: 9)
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not able to ligate PCR product into a vector... - what do you suggest? (reply: 2)
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Digestion and ligation problem - Insertion of 4Kb DNA fragment into a 10Kb vector (reply: 2)
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Another Problematic Ligation - 110bp ligation into 2.5kb vector (reply: 7)
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ligation, transformation problem - (reply: 8)
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A basic question about ligation - (reply: 3)
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Do I need CIP before my ligation - (reply: 4)
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Blunt ended ligation - (reply: 7)
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Transformation and ligation problems - (reply: 3)
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ligation problems - (reply: 8)
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how to confirm if ligation is sucessful? - (reply: 4)
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To inactivate or not to inactivate ligase before transformation? - (reply: 5)
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Please help me! my ligation is fail - (reply: 6)
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How to calculate DNA concentration for ligation - (reply: 8)
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ligation transformation of puc18 and phage lambda using ecor1 and bamh1 - general double/single digest problem (reply: 3)
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PCR of ligation products - (reply: 8)
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over time ligation - (reply: 5)
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Troubleshooting ligation/transformation - (reply: 1)
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Ligation/Transformation problem - (reply: 4)
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Ligation help needed! - vector religation? (reply: 3)
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Incubation conditions of T4 DNA ligase - (reply: 4)
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how to improve ligation efficiency - (reply: 7)
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What is a three-way ligation - (reply: 1)
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Ligation is killing me - 1kb insert, 6 kb vector (reply: 11)
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strange ligation problem - (reply: 1)
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ligation to transformation - (reply: 1)
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Ligation help ! - blunt end ligation probs. (reply: 4)
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Ligation of large DNA fragments (40 kb + 10 kb) - Does anyone have any suggestions? (reply: 2)
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Vector self ligation? - (reply: 1)
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Double Ligation - (reply: 8)
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ligating small oligos - how to ligate small oligos into vector (reply: 2)
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ligation question - (reply: 6)
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Ligation problem - (reply: 1)
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ligation of short oligos - (reply: 3)
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How to increase the efficacy of ligation when using single RE digestion - (reply: 17)
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three way ligation (two fragment and a vector) - (reply: 1)
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Ligation at 16°C - problems wit ligation (reply: 6)
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Fast ligation - (reply: 8)
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4 versus 16 degree for ligation reaction - (reply: 3)
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seemingly easy ligations don't work - (reply: 11)
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pcr ligation - (reply: 2)
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double enzyme cut and ligation - (reply: 3)
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Ligation woes - ligation? what's wrong (reply: 2)
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problems with same enzyme cutted DNAs ligation - (reply: 1)
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Calculate insert/vector ratio for ligation - (reply: 8)
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Ligation reaction - temperature and duration consideration - (reply: 5)
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ligation/transformation issue - (reply: 5)
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pBluescript ligation issues - pBS + thymidine kinase ligation (reply: 3)
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Double digestion/ligation problem - (reply: 4)
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Help on ligation of big fragment(9kb). - ideas on good ligases? (reply: 2)
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Is it possible to gel purify the ligated product? - Avoid non cut vector or self-liagted vector contam (reply: 2)
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Double digestion of PCR product and ligation - (reply: 5)
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ligation problem - (reply: 3)
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Can you store bacteria for ligation? - (reply: 5)
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ligation problem of double digested products - (reply: 3)
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DNA construction not working--is prep poisoning ligation? - DNA preparation/ligation problems (reply: 5)
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help: three fragment ligation - (reply: 1)
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Ligation issues - (reply: 1)
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ligation problem.. - (reply: 3)
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PPT in T4 DNA ligase buffer - (reply: 5)
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trouble shooting ligation and transform - (reply: 2)
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difficult ligation/transformation - please help (reply: 11)
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Has my ligation work? - (reply: 7)
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Blunt-Cohesive ends ligation - Blunt-Cohesive ends ligation (reply: 2)
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Ligation problems - (reply: 2)
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Ligation of two oligos into a vector - (reply: 4)
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Is it ok to store a digested vector for ligation? - (reply: 4)
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three fragments ligation problems - emergent help needed (reply: 2)
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should we dilute the DNA (vector and insert), before ligation? - ligation problem (reply: 5)
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too low DNA conc. after ligation..help! - (reply: 1)
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ligation problem - (reply: 6)
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Ligate two fragments with only one sticky end - (reply: 1)
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double restriction digestion - no ligation (reply: 6)
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Ligation problems - (reply: 3)
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Ligation of 2 fragments and one vector - (reply: 7)
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ligation of 2 linear sticky fragments - (reply: 4)
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DNA concentration for ligation - (reply: 8)
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factors contribute to the ligation failure.. - (reply: 1)
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Double insert ligation? - (reply: 2)
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ATP in ligation reaction - ligation efficiency (reply: 3)
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antibiotics concentration for ligated plasmid - (reply: 3)
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Self ligation of vector - (reply: 2)
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Store ligation reaction for repeat transformation? - (reply: 3)
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Ligation optimization - (reply: 4)
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blunt-sticky ligation/transformation problem - (reply: 6)
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ligation problem and dephosphorylate matters.... - (reply: 4)
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Ligation time - (reply: 7)
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help with big DNA ligation - (reply: 6)
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Blunt End Ligation !Which god to pray? - (reply: 2)
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ligation problem.. - (reply: 3)
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problem with ligation with ClaI - Cloning (reply: 7)
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ligation problems - ligation problems (reply: 7)
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Problem in the ligation - (reply: 2)
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Can I PCR amplify a ligation product directly - (reply: 3)
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Why ligation reactions are done at 4C - Ligation of insert into vector (reply: 2)
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poor ligation of plasmid/oligo construct - (reply: 8)
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CIPping after Klenow blunting - Is CIP necessary in Blunt end ligation? (reply: 2)
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Ligation of PCR products - (reply: 1)
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Cloning, ligation, transformation with pET - Problem re-ligating with NotI (reply: 1)
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vector auto ligation problem - (reply: 1)
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T4 ligase - is the inactivation step necessary? - (reply: 4)
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ligation problem - self ligation of vector - (reply: 5)
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Ligate two restriction fragments of dsDNA - (reply: 6)
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Recombinant DNA - Problems with ligation (reply: 1)
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Unstable plasmid ?? - Deletions only after ligation ! (reply: 2)
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Help! Three way ligation including blunt-end oligo - (reply: 2)
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The amount of plasmid for ligation reaction - Help (reply: 2)
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blunt end ligation problems - (reply: 6)
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PCR Ligation cloning - (reply: 2)
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Cloning problem - Digestion, ligation and transformation (reply: 6)
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ligation question - ligation (reply: 3)
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ligation question - ligation of size-selected DNA (reply: 4)
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Vector- Insert Ratio in Ligation - (reply: 2)
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Tripple Ligation - (reply: 7)
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ligation and transformation problems! Urgent! - (reply: 20)
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ligation and transformation - (reply: 11)
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random ligation question?? - (reply: 1)
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Ligation - (reply: 5)
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ligation of pcr product - (reply: 4)
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ligation problem - (reply: 1)
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ligation problems and searh for map of pGW1 vector - (reply: 1)
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Why don't get the desired ligation - (reply: 1)
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Ligations suck. - (reply: 6)
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ligation issues - Ligation (reply: 9)
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Help: try ligation but see nothing from the gels - (reply: 9)
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ligation help - (reply: 13)
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ligation - stick 3 pieces together - (reply: 10)
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Impossibly small ligation products - Impossibly small ligation products (reply: 3)
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Simple Ligation/cloning questions - question on dNTPs and ligation protocol (reply: 1)
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How to ligate ds-oligos into a vector? - (reply: 48)
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Problematic ligation - problems with ligation (reply: 1)
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Ligation Reaction - (reply: 8)
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ligation troubles... - (reply: 2)
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Ligation with PLAFR - (reply: 1)
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Ligation into EGFP problems - Ligation into EGFP problems (reply: 5)
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Double digestion and ligation problem - (reply: 15)
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ligation again - (reply: 3)
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ligation problem - (reply: 2)
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pcr ligation - problems with ligation (reply: 1)
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Ligation protocol - (reply: 1)
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about ligation and transformation - anxiety (reply: 3)
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42bp fragment ligation to 4776bp vector - (reply: 3)
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Stupid Ligation - (reply: 1)
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ligase three fragments - ligase three fragments (reply: 3)
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Digestion of Plasmid - Xho-I digestion of Ligated product (reply: 1)
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3-fragment ligation - (reply: 3)
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Ligation in PTYB12 vectors ofthe IMPACT protein purification system... - (reply: 1)
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RNA in plasmid preparation interfere in ligation - RNA contamination and ligation (reply: 3)
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Any experience in improving blunt end ligation? - (reply: 6)
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Help with ligation into IMPACT-CN pTyb11! - (reply: 2)
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ligation - (reply: 1)
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rapid ligation - (reply: 1)
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about rapid ligation - (reply: 2)
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Ligation problems - (reply: 6)
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Ligation problems - (reply: 4)
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Met trouble in ligation! - insert:sacII,XhoI;vector:sacII,SalI (reply: 1)
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Failure of ligation - (reply: 1)
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ligation problem - (reply: 2)
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ligation problems - cloning a PCR fragment - into a double-cut vector (reply: 4)
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oligo hybridization and ligation - hybridization and ligation (reply: 1)
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Subgenomic library construction - ligation problems - (reply: 2)
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rapid ligation - (reply: 3)
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DNA ligation - (reply: 3)
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ligate 3 DNA fragments with sticky ends into Vector - (reply: 1)
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ligation problem with the insert size bigger than 4kb - (reply: 5)
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Ligations - (reply: 2)
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ligation - the problem of ligation? (reply: 4)
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RNA ligation - (reply: 3)
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TOPO ligation - (reply: 1)
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ligation problem - (reply: 5)
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Confused ligation - (reply: 4)