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31. cell lysis - (reply: 3)
32. help with mechanism - (reply: 2)
33. RAW 264 cells - (reply: 1)
34. Multi-spanning membrane protein - C terminal tagged (reply: 4)
35. Enzyme assay - (reply: 1)
36. Adenoviral purification by CsCl - Only cell pellet or supernatant too??? (reply: 5)
37. Does FRET by BFP/GFP work? - (reply: 2)
38. Where to get mouse peritonel macrophages - (reply: 1)
39. Heat-shock treatment problems with adherent cells - (reply: 1)
40. Determining if a protein is a downstream target of another protein - (reply: 4)
41. Can Osteosarcoma cells change their phenotype? - previously "Name this cancer cell's purpose and parts" (reply: 1)
42. Nutrient deficient medium - (reply: 1)
43. Cell stats - looking for info (reply: 1)
44. Problems with nucleus isolation and 293 cells - Nucleus seems to disrupt. What to do? (reply: 1)
45. Apoptosis vurses necrosis - Way to find wether the cell death is due to apoptosis or necrosis? (reply: 13)
46. Cell surface Protein Isolation - Protein extraction procedure (reply: 4)
47. Name this cancer cell's purpose and parts - (reply: 1)
48. 0.2-1% DMSO - (reply: 4)
49. Phagoyctosis assay and fluorescent beads - (reply: 3)
50. mixed glial cultures - to coat plates...? (reply: 2)
51. problem with 70% ethanol - (reply: 7)
52. Does it look like cell clumps/artefact/contamination? - (reply: 3)
53. What would happen if we don't use protease inhibitors? - for cell lysis by RIPA, NP40 or any other lysis buffer (reply: 8)
54. Starting a cell line - (reply: 6)
55. How to remove ice from door gasket of -80 freezer - (reply: 12)
56. what cell line to use for visualizing membrane localized protein - (reply: 1)
57. how to purify the primary preadipocyte culture? - many fibroblast contamination (reply: 4)
58. transfection - (reply: 1)
59. DNA "snot" in cell lysis/protein extraction - (reply: 6)
60. BD Invasion Chamber - (reply: 1)
32. help with mechanism - (reply: 2)
33. RAW 264 cells - (reply: 1)
34. Multi-spanning membrane protein - C terminal tagged (reply: 4)
35. Enzyme assay - (reply: 1)
36. Adenoviral purification by CsCl - Only cell pellet or supernatant too??? (reply: 5)
37. Does FRET by BFP/GFP work? - (reply: 2)
38. Where to get mouse peritonel macrophages - (reply: 1)
39. Heat-shock treatment problems with adherent cells - (reply: 1)
40. Determining if a protein is a downstream target of another protein - (reply: 4)
41. Can Osteosarcoma cells change their phenotype? - previously "Name this cancer cell's purpose and parts" (reply: 1)
42. Nutrient deficient medium - (reply: 1)
43. Cell stats - looking for info (reply: 1)
44. Problems with nucleus isolation and 293 cells - Nucleus seems to disrupt. What to do? (reply: 1)
45. Apoptosis vurses necrosis - Way to find wether the cell death is due to apoptosis or necrosis? (reply: 13)
46. Cell surface Protein Isolation - Protein extraction procedure (reply: 4)
47. Name this cancer cell's purpose and parts - (reply: 1)
48. 0.2-1% DMSO - (reply: 4)
49. Phagoyctosis assay and fluorescent beads - (reply: 3)
50. mixed glial cultures - to coat plates...? (reply: 2)
51. problem with 70% ethanol - (reply: 7)
52. Does it look like cell clumps/artefact/contamination? - (reply: 3)
53. What would happen if we don't use protease inhibitors? - for cell lysis by RIPA, NP40 or any other lysis buffer (reply: 8)
54. Starting a cell line - (reply: 6)
55. How to remove ice from door gasket of -80 freezer - (reply: 12)
56. what cell line to use for visualizing membrane localized protein - (reply: 1)
57. how to purify the primary preadipocyte culture? - many fibroblast contamination (reply: 4)
58. transfection - (reply: 1)
59. DNA "snot" in cell lysis/protein extraction - (reply: 6)
60. BD Invasion Chamber - (reply: 1)