mixed glial cultures - to coat plates...? (Oct/14/2008 )

Hi,

I am growing mixed glial cultures, and I thought they would adhere to the wells in my 24 well plate, but only some have... does anyone else do this? WOuld you suggest coating the wells with poly-D or should the astrocytes and microglia plate down to the bottom of the well with out the poly L or Poly D?

I coat my coverslips when using the cells for immuno but someone said I do not need to coat the wells? Anyone any ideas,

thanks!

-scistudent-

QUOTE (scistudent @ Oct 14 2008, 03:02 AM)

in a couple of days both will adhere .

However i strongly recommend using Poly-lysine (PLL is much cheaper than PDL and works fine, you can also use Poly-L-ornithone)

-Lego-

Thanks,

The cells seemed to form spheres and then some did adhere and some didnt. Some of the spheres adhered and then cells grew outward... Next time I'll coat the wells! so Im just going to try save the cells for PCR, as I figure I cant do an ELISA on them due to the differing cell number of cells in each well.

-scistudent-