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1. How to prevent cell aggregation - (reply: 1)
2. Isolation of lymphocytes from mouse stomach. Major help needed. - (reply: 5)
3. Measure intracelluar ROS - Protocol for DCF-DA staining? (reply: 2)
4. OK to freeze lysates for assay later? - (reply: 1)
5. What factors affect the quality of image obtained using a light microscope? How - Answers highly appreciated... (reply: 4)
6. How to stimulate Abl kinase activity? - (reply: 2)
7. Photosynthesis question - (reply: 1)
8. boosting expression in stable cell line? - (reply: 5)
9. too few cells used for electroporation/ transfection - (reply: 4)
10. VEGF secretion in MDA-MB-231 cell line - (reply: 3)
11. Quantification of VEGF peptide levels in human breast cancer cells. HELP! - (reply: 6)
12. Yeast transformation - low efficiency (reply: 2)
13. can't detect bcl-2 in cytosol - but Bax is there! (reply: 2)
14. contransfection why choose beta galactocidase? - why not an antibiotic resistant plasmid (reply: 5)
15. Cell pellet after freezing - (reply: 3)
16. Treating cells with cytokines - Should you take out growth factors before treating? (reply: 2)
17. Which part of the kidney are COS-7 cells from? - (reply: 1)
18. Ways to stop the cell at G1 phase? - (reply: 2)
19. co-transfgection - antibiotic plasmid instead of B galactocidase (reply: 2)
20. difference between transformed and immortalized cells - (reply: 3)
21. Is it hard to make knock-out cells/mice? - (reply: 4)
22. How to avoid the formation of bubbles when using pipette? - (reply: 4)
23. How do cellular fractions look like on SDS-PAGE gel? - what bands am I supposed to see in different fractions? (reply: 2)
24. How to calculate doubling time - (reply: 2)
25. Absorbance for MTT - What are the ranges of wavelengths for MTT detection and background? (reply: 4)
26. Seletion marker - functional in the ER lumen (reply: 2)
27. HUVECs - cells detach after 2 days (reply: 3)
28. What is "osmotic support"? - (reply: 2)
29. What color should the mitochondrial pellet be? - I got a white pellet after subcellular fractionation (reply: 2)
30. anybody has experience in alternative splicing? - (reply: 2)
2. Isolation of lymphocytes from mouse stomach. Major help needed. - (reply: 5)
3. Measure intracelluar ROS - Protocol for DCF-DA staining? (reply: 2)
4. OK to freeze lysates for assay later? - (reply: 1)
5. What factors affect the quality of image obtained using a light microscope? How - Answers highly appreciated... (reply: 4)
6. How to stimulate Abl kinase activity? - (reply: 2)
7. Photosynthesis question - (reply: 1)
8. boosting expression in stable cell line? - (reply: 5)
9. too few cells used for electroporation/ transfection - (reply: 4)
10. VEGF secretion in MDA-MB-231 cell line - (reply: 3)
11. Quantification of VEGF peptide levels in human breast cancer cells. HELP! - (reply: 6)
12. Yeast transformation - low efficiency (reply: 2)
13. can't detect bcl-2 in cytosol - but Bax is there! (reply: 2)
14. contransfection why choose beta galactocidase? - why not an antibiotic resistant plasmid (reply: 5)
15. Cell pellet after freezing - (reply: 3)
16. Treating cells with cytokines - Should you take out growth factors before treating? (reply: 2)
17. Which part of the kidney are COS-7 cells from? - (reply: 1)
18. Ways to stop the cell at G1 phase? - (reply: 2)
19. co-transfgection - antibiotic plasmid instead of B galactocidase (reply: 2)
20. difference between transformed and immortalized cells - (reply: 3)
21. Is it hard to make knock-out cells/mice? - (reply: 4)
22. How to avoid the formation of bubbles when using pipette? - (reply: 4)
23. How do cellular fractions look like on SDS-PAGE gel? - what bands am I supposed to see in different fractions? (reply: 2)
24. How to calculate doubling time - (reply: 2)
25. Absorbance for MTT - What are the ranges of wavelengths for MTT detection and background? (reply: 4)
26. Seletion marker - functional in the ER lumen (reply: 2)
27. HUVECs - cells detach after 2 days (reply: 3)
28. What is "osmotic support"? - (reply: 2)
29. What color should the mitochondrial pellet be? - I got a white pellet after subcellular fractionation (reply: 2)
30. anybody has experience in alternative splicing? - (reply: 2)