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1981. Eliminating background staining for ICC - ICC (reply: 1)
1982. Measuring nitrite from LPS-activated RAW264.7 cells - (reply: 10)
1983. MEM or DMEM - why sometime MEM and sometime DMEM? (reply: 1)
1984. following fusion in real time? - (reply: 2)
1985. Help with defective cell lines - (reply: 1)
1986. How to detect apoptosis?? - (reply: 4)
1987. Scraping or Trypsinization for westerns?? - (reply: 10)
1988. about penicilin streptomycin - (reply: 6)
1989. How to measure neuronal cell differentiation? - (reply: 1)
1990. Stable transfection of S2R+ and Clone8 cells - (reply: 1)
1991. Cell Fusion - Fusing spleen cells with myeloma cells using PEG (reply: 1)
1992. Stable transfection - When to start selection? (reply: 4)
1993. How to do transfection on 293 cell - (reply: 4)
1994. Organ Culture - (reply: 4)
1995. Cell Death. optical spillovers.alternative assay - Need an assay for apoptosis (reply: 1)
1996. Immunocytochemistry for identifying vascular smooth muscle cells - (reply: 2)
1997. Fixing cells for performing immunoflourescense? - Help with protocol (reply: 3)
1998. NF-kB in transient reporter gene assay - (reply: 1)
1999. liquid nitrogen storage - gas or liquid (reply: 2)
2000. Difference between cell proliferation and cytotoxicity kits? - (reply: 2)
2001. Reviving dead glycerol archive - (reply: 3)
2002. pGen.IRES.GFP.Neo - (reply: 3)
2003. G418 in freezing medium? - (reply: 2)
2004. How to obtain a stable transfectant - (reply: 4)
2005. transfection - selection of trransfected SH-SY5Y cells (reply: 3)
2006. Tripsin EDTA detachment - doubt (reply: 4)
2007. where is the mycoplasma from?? - (reply: 9)
2008. Need help with transfection before get crazy - Primary human lymphocytes (reply: 2)
2009. Who killed my cells??? - Toxicity during transfection (reply: 3)
2010. plasmid transfection and virus infection simutaneously - (reply: 2)
1982. Measuring nitrite from LPS-activated RAW264.7 cells - (reply: 10)
1983. MEM or DMEM - why sometime MEM and sometime DMEM? (reply: 1)
1984. following fusion in real time? - (reply: 2)
1985. Help with defective cell lines - (reply: 1)
1986. How to detect apoptosis?? - (reply: 4)
1987. Scraping or Trypsinization for westerns?? - (reply: 10)
1988. about penicilin streptomycin - (reply: 6)
1989. How to measure neuronal cell differentiation? - (reply: 1)
1990. Stable transfection of S2R+ and Clone8 cells - (reply: 1)
1991. Cell Fusion - Fusing spleen cells with myeloma cells using PEG (reply: 1)
1992. Stable transfection - When to start selection? (reply: 4)
1993. How to do transfection on 293 cell - (reply: 4)
1994. Organ Culture - (reply: 4)
1995. Cell Death. optical spillovers.alternative assay - Need an assay for apoptosis (reply: 1)
1996. Immunocytochemistry for identifying vascular smooth muscle cells - (reply: 2)
1997. Fixing cells for performing immunoflourescense? - Help with protocol (reply: 3)
1998. NF-kB in transient reporter gene assay - (reply: 1)
1999. liquid nitrogen storage - gas or liquid (reply: 2)
2000. Difference between cell proliferation and cytotoxicity kits? - (reply: 2)
2001. Reviving dead glycerol archive - (reply: 3)
2002. pGen.IRES.GFP.Neo - (reply: 3)
2003. G418 in freezing medium? - (reply: 2)
2004. How to obtain a stable transfectant - (reply: 4)
2005. transfection - selection of trransfected SH-SY5Y cells (reply: 3)
2006. Tripsin EDTA detachment - doubt (reply: 4)
2007. where is the mycoplasma from?? - (reply: 9)
2008. Need help with transfection before get crazy - Primary human lymphocytes (reply: 2)
2009. Who killed my cells??? - Toxicity during transfection (reply: 3)
2010. plasmid transfection and virus infection simutaneously - (reply: 2)