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1951. How to reduce cell clumping for immunocytostaining - (reply: 1)
1952. Isolation of outer membrane protein - Protein isolation (reply: 3)
1953. Websites or books about stem cell culture - (reply: 2)
1954. primary culture transfection problems - any transfection methods on primary granulosa cell (reply: 1)
1955. Help: mitochondria extraction - (reply: 1)
1956. ATCC cell lines - How is your experience? (reply: 1)
1957. Extensive Cell Death with Serum Free Media - How to Avoid Extensive Cell Death with Serum Free (reply: 8)
1958. growing hybridomas - (reply: 1)
1959. PC3 cell line - Confused (reply: 8)
1960. poly-L- vs. poly-D-lisine - (reply: 1)
1961. storing blood from mouse - Can blood be stored, and if yes, How? (reply: 2)
1962. Transfection, GFP, FACS and UV Microscope - great inconsistency b/t FACS and UV microscope (reply: 3)
1963. questions about adherent cells - they are detaching (reply: 4)
1964. Cell permeabilisation strategies - (reply: 1)
1965. primary culture - measuring transcription - mRNA expression in primary culture (reply: 1)
1966. PCR primers for Mycoplasma detection? - Publication gives many, which to choose? (reply: 1)
1967. why does DMEM pH increase - Tissue culture (reply: 2)
1968. reporter assays - luciferase reporter assay (reply: 2)
1969. Silly question: is DMSO sterile by itself, to add it to cells? - Lab stock bottle could be used? (reply: 6)
1970. paraformaldehyde to IFA - (reply: 3)
1971. How to grow adherent cells without attachment??? - (reply: 2)
1972. Cell clumping during thawing - (reply: 2)
1973. mcf-7 proliferation - (reply: 2)
1974. EtBr problem - (reply: 1)
1975. Is L-glutamine easily degradale in RPMI1640? - (reply: 3)
1976. Adenovirus culture/isolation - (reply: 3)
1977. Southern Blotting - (reply: 2)
1978. Reference to Cheeney buffer - (reply: 1)
1979. contamination of cell culture by another cell type - (reply: 1)
1980. Lysis buffer - muscle cells lysis (reply: 1)
1952. Isolation of outer membrane protein - Protein isolation (reply: 3)
1953. Websites or books about stem cell culture - (reply: 2)
1954. primary culture transfection problems - any transfection methods on primary granulosa cell (reply: 1)
1955. Help: mitochondria extraction - (reply: 1)
1956. ATCC cell lines - How is your experience? (reply: 1)
1957. Extensive Cell Death with Serum Free Media - How to Avoid Extensive Cell Death with Serum Free (reply: 8)
1958. growing hybridomas - (reply: 1)
1959. PC3 cell line - Confused (reply: 8)
1960. poly-L- vs. poly-D-lisine - (reply: 1)
1961. storing blood from mouse - Can blood be stored, and if yes, How? (reply: 2)
1962. Transfection, GFP, FACS and UV Microscope - great inconsistency b/t FACS and UV microscope (reply: 3)
1963. questions about adherent cells - they are detaching (reply: 4)
1964. Cell permeabilisation strategies - (reply: 1)
1965. primary culture - measuring transcription - mRNA expression in primary culture (reply: 1)
1966. PCR primers for Mycoplasma detection? - Publication gives many, which to choose? (reply: 1)
1967. why does DMEM pH increase - Tissue culture (reply: 2)
1968. reporter assays - luciferase reporter assay (reply: 2)
1969. Silly question: is DMSO sterile by itself, to add it to cells? - Lab stock bottle could be used? (reply: 6)
1970. paraformaldehyde to IFA - (reply: 3)
1971. How to grow adherent cells without attachment??? - (reply: 2)
1972. Cell clumping during thawing - (reply: 2)
1973. mcf-7 proliferation - (reply: 2)
1974. EtBr problem - (reply: 1)
1975. Is L-glutamine easily degradale in RPMI1640? - (reply: 3)
1976. Adenovirus culture/isolation - (reply: 3)
1977. Southern Blotting - (reply: 2)
1978. Reference to Cheeney buffer - (reply: 1)
1979. contamination of cell culture by another cell type - (reply: 1)
1980. Lysis buffer - muscle cells lysis (reply: 1)