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541. Plasmid replication in mammalian cell systems - (reply: 3)
542. The secret? cytosolic proteins adherent macrophages? - (reply: 1)
543. transfection reagent for HUVECs ? - high cytotoxicity problem (reply: 2)
544. yield of PBMCs - (reply: 2)
545. reporter assay interpretation - repressor overexpression (reply: 2)
546. How much serum should I put in the tissue culture - (reply: 5)
547. Some astrocytes don´t attach... - ...but others do (reply: 2)
548. Sodium Orthovanadate kinetics in media incubations - (reply: 3)
549. the outer wells - (reply: 4)
550. MTT assay + suspension cells - (reply: 4)
551. need help, my collagen gels are watery - (reply: 1)
552. AML cell lines - Where can I get them?? (reply: 1)
553. kinase - kinase assay (reply: 2)
554. Clumping of normal colon cells due to transfection reagent - Counting problems (reply: 6)
555. CFP YFP - relocation (reply: 3)
556. why my suspension cells changed to adherent cells? - (reply: 3)
557. CO2 monitor for incubator - What do you guys do? - Suspicious cell stress/death (reply: 4)
558. 3H Thymidine assay with non-adherent cells / cell harvester - cell harvester a must? alternatives? (reply: 2)
559. Redox sensitive dyes - measuring ROS (reply: 1)
560. plating caco-2 cells - Caco-2 cells don't form monolayer and MEM turned pink!! why?? (reply: 2)
561. Determining the RT concentration in HIV-1 samples through Sandwich ELISA - Not seeing any infection/RT activity/indication of virus (reply: 7)
562. Recommendations - - cheap, quick and cell safe Rnase a inhibitor/inactivator - (reply: 1)
563. Trypan blue & methylene blue - both to measure cell viability? (reply: 2)
564. stability of doxycycline - (reply: 3)
565. Just how many cells are lost when changing tubes? - (reply: 5)
566. collect secreted proteins in cell culture media - (reply: 2)
567. Where exactly are mesenchymal stem cells located in bone marrow? - (reply: 1)
568. problem with plating adherent cells in 24-well plates - (reply: 11)
569. Jurkat/J77 cell line specifics - (reply: 2)
570. Who makes 200-350cm2 tissue culture roller bottles? - (reply: 8)
542. The secret? cytosolic proteins adherent macrophages? - (reply: 1)
543. transfection reagent for HUVECs ? - high cytotoxicity problem (reply: 2)
544. yield of PBMCs - (reply: 2)
545. reporter assay interpretation - repressor overexpression (reply: 2)
546. How much serum should I put in the tissue culture - (reply: 5)
547. Some astrocytes don´t attach... - ...but others do (reply: 2)
548. Sodium Orthovanadate kinetics in media incubations - (reply: 3)
549. the outer wells - (reply: 4)
550. MTT assay + suspension cells - (reply: 4)
551. need help, my collagen gels are watery - (reply: 1)
552. AML cell lines - Where can I get them?? (reply: 1)
553. kinase - kinase assay (reply: 2)
554. Clumping of normal colon cells due to transfection reagent - Counting problems (reply: 6)
555. CFP YFP - relocation (reply: 3)
556. why my suspension cells changed to adherent cells? - (reply: 3)
557. CO2 monitor for incubator - What do you guys do? - Suspicious cell stress/death (reply: 4)
558. 3H Thymidine assay with non-adherent cells / cell harvester - cell harvester a must? alternatives? (reply: 2)
559. Redox sensitive dyes - measuring ROS (reply: 1)
560. plating caco-2 cells - Caco-2 cells don't form monolayer and MEM turned pink!! why?? (reply: 2)
561. Determining the RT concentration in HIV-1 samples through Sandwich ELISA - Not seeing any infection/RT activity/indication of virus (reply: 7)
562. Recommendations - - cheap, quick and cell safe Rnase a inhibitor/inactivator - (reply: 1)
563. Trypan blue & methylene blue - both to measure cell viability? (reply: 2)
564. stability of doxycycline - (reply: 3)
565. Just how many cells are lost when changing tubes? - (reply: 5)
566. collect secreted proteins in cell culture media - (reply: 2)
567. Where exactly are mesenchymal stem cells located in bone marrow? - (reply: 1)
568. problem with plating adherent cells in 24-well plates - (reply: 11)
569. Jurkat/J77 cell line specifics - (reply: 2)
570. Who makes 200-350cm2 tissue culture roller bottles? - (reply: 8)