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Membrane Related Discussions
  1. False characterization of membrane transporter type - (reply: 1)
  2. EGFP cloning and membrane protein - (reply: 1)
  3. How long a membrane can be saved stripping? - (reply: 3)
  4. resting membrane potential - (reply: 3)
  5. Multi-spanning membrane protein - C terminal tagged (reply: 4)
  6. Membrane protein problem - Problem in getting signals (reply: 3)
  7. membrane filter - (reply: 2)
  8. protein on membrane - (reply: 1)
  9. westerm Blot membrane - (reply: 4)
  10. what cell line to use for visualizing membrane localized protein - (reply: 1)
  11. what cell line to use for visualizing membrane localized protein - (reply: 1)
  12. Smeared bands for MIDDLE SECTION of the membrane - (reply: 7)
  13. ultracentrifugation in membrane protein extraction - (reply: 2)
  14. plasma membrane tag - (reply: 2)
  15. Separation of cytosol and membrane proteins by (ultra)centrifugation - (reply: 5)
  16. Trying to detect correct membrane protein size in gel - At wits end (reply: 4)
  17. transfer problem - i cannot transfer proteins to a pvdf membrane (reply: 7)
  18. membrane striping - (reply: 1)
  19. Checking purity of plasma membrane fraction - (reply: 8)
  20. NP40 can be used for lysis nulear membrane? - (reply: 3)
  21. membrane protein extraction - membrane protein extraction (reply: 7)
  22. membrane protein and boiling - (reply: 3)
  23. Smeary western blots of cell lysates - western of a HMW membrane protein (reply: 2)
  24. Measuring Membrane Potentials - (reply: 1)
  25. HELP MEMBRANE DEPLOARIZATION AND DIBAC4(3) INVITROGEN - DiBAC4(3)?? (reply: 2)
  26. Stripping of dry membrane? - can we do that? (reply: 2)
  27. IF - Methanol Vs. Triton? - Cytoskeleton and Membrane associated proteins (reply: 2)
  28. transfering proteins from 2-D gel to nitrocellulose membrane - (reply: 5)
  29. Membrane protein - (reply: 11)
  30. Fixation to PVDF membranes - (reply: 2)
  31. Extracting only plasma membrane - (reply: 5)
  32. Can I incubate membrane with 2 antibodies at the same time ? - (reply: 5)
  33. how to solubilize out functional membrane protein - (reply: 4)
  34. Membrane Froze - Did this ruin my results? - Western Blot not working anymore?? (reply: 2)
  35. boiling membranes - (reply: 4)
  36. microsomal membrane isolation - (reply: 1)
  37. Circular cassette imprint on membrane - (reply: 2)
  38. membrane lipids - (reply: 3)
  39. Protein into the membrane - (reply: 2)
  40. Agent to punch hole in cell membranes - (reply: 1)
  41. protein an its phospho-protein in the same membrane - (reply: 3)
  42. marker proteins for plasm membrane in c2c12 myoblasts - (reply: 1)
  43. membrane stripping methods - (reply: 1)
  44. How to prepare membranes from E.coli? - (reply: 2)
  45. Membrane protein extraction from mice tissue - (reply: 3)
  46. Membrane Protein Extraction and Analysis Questions - (reply: 1)
  47. Western blotting for membrane bounded protein - (reply: 7)
  48. ER membrane prep for 2D gels - liver micrsosomes (reply: 4)
  49. How can I resuspend membrane proteins for further inmunizations? - (reply: 1)
  50. Western for membrane bound protein - (reply: 11)
  51. reusing PVDF membrane for different antibodies without stripping - (reply: 6)
  52. Immonuprecipitation of membrane proteins, is it possible? - (reply: 1)
  53. detecting small membrane protein by western blot - (reply: 4)
  54. Nitrocellulose or PVDF membrane for western blot - which one???? (reply: 8)
  55. Ultrafiltration Membrane Cloggged - (reply: 3)
  56. wetern for membrane associated protein - (reply: 1)
  57. can detet anything with my western membrane - (reply: 8)
  58. Does trypsin digest integral plasma membrane proteins? - (reply: 2)
  59. question on membrane extraction protocol - (reply: 1)
  60. Storing a NC membrane that has yet to be processed - (reply: 2)
  61. lysis buffer for coip with a membrane protein - (reply: 2)
  62. Problems with DIG DNA labeling! help! - Membrane was completely stained purple with no bands (reply: 12)
  63. Western Blot: Re-use of membrane? - (reply: 4)
  64. Western problem: bubbles forming INSIDE PVDF membrane - (reply: 4)
  65. storing membranes - (reply: 3)
  66. Amicon membrane integrity - (reply: 2)
  67. Bloting IEF gels onto PVDF membranes? - (reply: 1)
  68. Crosslinking of oligos to membrane required for EMSA? - Is it necessary to crosslink the oligos to a positively charged nylon (reply: 2)
  69. western blotting transfer onto membrane:why does current increase? - At constant voltage, resistance increases. The current also increases. (reply: 5)
  70. Membrane proteins extraction protocol - (reply: 2)
  71. Can membrane proteins be separated using 2D-PAGE?!?! - (reply: 2)
  72. protocal for membrane extraction/fractionation - (reply: 3)
  73. membrane protein western blot - (reply: 3)
  74. loading control for membrane fraction - beta-actin? (reply: 4)
  75. Shock--nothing at all after washing membrane in northern blotting - (reply: 2)
  76. membrane isolation protocol - (reply: 1)
  77. re-probing membrane - (reply: 6)
  78. Adherence of Candida to Saliva Free Mucous Membranes - (reply: 2)
  79. why did the membrane protein disapear again? - (reply: 3)
  80. Isolation membrane proteins in "cell-friendly" buffer - extracted proteins need to be tested in in vitro models (reply: 1)
  81. Transfer optimization of membrane proteins to PVDF - (reply: 3)
  82. Does Ponceau stain of NC membrane interfere with Ab binding? - (reply: 4)
  83. Membrane of Qiaquick remain coloured - Problem of elution - (reply: 1)
  84. Western Blot membrane disintegration - (reply: 5)
  85. 2DE of hydrophobic membrane protein (problem) - (reply: 3)
  86. How many times can one strip a membrane? - (reply: 2)
  87. cell membrane - (reply: 6)
  88. Removing serum proteins bound to Plasma membrane? - (reply: 9)
  89. storage of membranes - (reply: 2)
  90. Protein extraction from soya meal using membrane - (reply: 1)
  91. Histidine in buffer, what is the role? - cytosolic and membrane protein isolation (reply: 1)
  92. Will Yeast2Hybrid work on membrane proteins - (reply: 2)
  93. Stripping membrane of Western blots - Introduction (reply: 10)
  94. keep membranes freezed? - (reply: 9)
  95. inverted inner membrane vesicle - (reply: 2)
  96. Membrane isolation - (reply: 1)
  97. A question on membrane filter assay - (reply: 2)
  98. I want to strip the protein off PVDF membrane - (reply: 4)
  99. can we purify the protein from westernblot membrane? - (reply: 5)
  100. What molecular weight cutoff dialysis membrane to use for virus concentration - (reply: 11)
  101. the voltage or current of transfer membrane for 200~300 kd protein - (reply: 3)
  102. Disadvantages of membrane stripping and reprobing - I need your opinion ASAP (reply: 2)
  103. Stripping membrane - (reply: 3)
  104. how can i use SDS and Triton X-100 to isolate the membrane protein? - (reply: 6)
  105. HOw can i isolate the membrane protein? - (reply: 9)
  106. Glycoprotein sequencing & PVDF membrane transfer - (reply: 3)
  107. cell lysis buffer for membrane protein please? - desperately need help.... :S (reply: 8)
  108. How do you purify membrane protein? - help (reply: 2)
  109. Protein immobilization on nitrocellulose membrane - (reply: 4)
  110. How long can a protein last on membrane? - (reply: 10)
  111. Array antibodies onto membranes - (reply: 4)
  112. Need prototocol for membrane fractionation from mammalian cells - (reply: 1)
  113. Burned membrane?.... - (reply: 1)
  114. Storing PVDF membranes for N-terminal sequencing - Urgent! (reply: 1)
  115. Membrane protein extraction - What about MEM-PER kit (Pierce)? (reply: 1)
  116. Membrane protein extraction - Qiagen extraction kit? (reply: 2)
  117. membrane protein aggregation - (reply: 2)
  118. Membrane protein extraction and detection - General lab tech (reply: 1)
  119. How to bake membranes without detergent ? - (reply: 5)
  120. reprobing old membrane without stripping - (reply: 5)
  121. GM1 detection on blotted membrane - (reply: 2)
  122. Unequal signal intensity across Western blot membrane - (reply: 4)
  123. How to strip PVDF membrane after imaging ? - For western blot (reply: 2)
  124. western blotting membranes - (reply: 6)
  125. Western blotting without transfering protein to membrane - (reply: 4)
  126. Membrane potentials - (reply: 1)
  127. How to get rid of radioactive probe from nylon membrane? - (reply: 7)
  128. What do you do with YOUR membranes? - (reply: 7)
  129. effect of enzymes on cell membrane - (reply: 4)
  130. cloning membrane protein with 2 tags - (reply: 2)
  131. protein tranfer from gel to membrane, reversible ? - (reply: 2)
  132. faint ladder on my membrane. Can hardly see it! - (reply: 9)
  133. Outer membrane protein in the supernatant!? - (reply: 1)
  134. Denature proteins on the membrane - (reply: 4)
  135. What is the transport mechanism of lipophilic drugs/agents into a cell? - Cell Membrane & Apoptosis (reply: 3)
  136. Is membrane protein always insoluble? - (reply: 2)
  137. Membrane protein purification - (reply: 1)
  138. transfer through the PVDF membrane - (reply: 5)
  139. Nylon membranes - (reply: 9)
  140. which part is the synovial membrane of the rat? - (reply: 3)
  141. Immobilon PSQ membrane - high background - (reply: 4)
  142. membrane protein western blot - (reply: 2)
  143. westernblot problem! - membrane protein! (reply: 2)
  144. western blot problem - antibodies can't detect the protein on the membrane (reply: 3)
  145. detection of phosphorylated proteins on WB - how stable is phosphorylation once transferred on WB membrane? (reply: 6)
  146. membrane filter technique (fecal coliform) - equipment and lab manual help (reply: 1)
  147. Killing of cells via the membrane - (reply: 4)
  148. Plasmatic cell membrane isolation (URGENT!) - (reply: 3)
  149. How to preserve southern membranes - (reply: 6)
  150. membrane protein extraction from dissected thymus - (reply: 1)
  151. immunoprecipitation of membrane protein from gram+ bacteria - (reply: 2)
  152. Plasma membrane marker - (reply: 2)
  153. membrane protein - (reply: 1)
  154. Storing PVDF membrane Help! - (reply: 4)
  155. IP the membrane protein - How to elute the protein complex from the beads (reply: 2)
  156. Western Blot troubleshooting: band is too high - LiCor imaging of Alpha tubulin membranes (reply: 6)
  157. Cell Membrane Permeability - (reply: 1)
  158. Cell membrane marker - (reply: 1)
  159. Plasma membrane marker - (reply: 1)
  160. Lab dealing with factors affecting cell membrane permeability - (reply: 1)
  161. extraction of membrane protiens - protocol for extraction of membrane bound protein? (reply: 6)
  162. Isolation of outer membrane protein - Protein isolation (reply: 3)
  163. Purifying a membrane protein - - the trouble with lipids? (reply: 3)
  164. What is the role of integral membrane proteins in signaling? - (reply: 1)
  165. problem with stripping membranes of western blott - do you know some efficient stripping buffer (reply: 3)
  166. WB for Membrane Protein - Not able to see membrane protein on western (reply: 12)
  167. WB for large proteins - Transfer conditions with PVDF membranes (reply: 5)
  168. Lysis buffer for membrane protien - (reply: 1)
  169. PVDF vs. nitrocellulose membrane - (reply: 4)
  170. Stripping membranes for WB - To strip or not to strip... ? (reply: 9)
  171. Western Blot question: cutting membrane into strips - (reply: 2)
  172. prepartion of membrane protein - (reply: 3)
  173. problems with membrane fraction - (reply: 2)
  174. Dry membrane to do western blotting - (reply: 4)
  175. About skim milk for blocking the membrane - (reply: 7)
  176. membrane fraction extraction for PKC - (reply: 2)
  177. How to prevent membrane bound receptor from endocytosis - (reply: 2)
  178. SDS-PAGE gel sticking - Gel sticking to the membrane (reply: 3)
  179. expression of fusion protein - how to lysis membrane protein (reply: 2)
  180. membrane and mitochondrial protein fractionation - (reply: 1)
  181. Storing a protein membrane overnight? - (reply: 5)
  182. coomassie blue stained gel - possible to obtain good blotting onto membrane? (reply: 3)
  183. SDS-PAGE of protein from membrane fraction - problems with protein band profile (reply: 1)
  184. membrane protein purification - solubilization and detergent removal (reply: 2)
  185. Putting more than one membrane in a container... - Western (reply: 2)
  186. Western for membrane bound receptor - (reply: 1)
  187. Amersham Hybond-P membrane and small protein - (reply: 2)
  188. membrane protein in SDS-PAGE ! - (reply: 3)
  189. Membrane Protein Western Blot. - Troubleshooting western blot (reply: 4)
  190. antibodies against membrane proteins - detection failure (reply: 3)
  191. Extraction of membrane proteins and western on them - (reply: 3)
  192. I forgot to pretreat my dialysis membrane ! - (reply: 1)
  193. I forgot pretreating the dialysis membrane ! - (reply: 1)
  194. problem on membrane preparation - (reply: 1)
  195. multilayer membranes for Westerns - looking for a product (reply: 1)
  196. Membrane protein isolation - (reply: 1)
  197. western blot for membrane protein with his-tag - can't detect with anti-his tag Ab? (reply: 3)
  198. Purification of a cell membrane protein - (reply: 1)
  199. Isolation of cell membrane - (reply: 5)
  200. Palsma membrane vesicles - (reply: 1)
  201. Proteins and Membranes - (reply: 2)
  202. Immunoprecipitation with embryo membrane protein - Looking for a protocol (reply: 1)
  203. membrane protein - (reply: 2)
  204. cDNA library (in Lambda  ZAP vector) screening - picking up plaques on nitro cellulose  membrane as replica (reply: 2)
  205. membrane protein purification - how to pufify cd3 protein from jurkat cell (reply: 1)
  206. Methods needed (E.coli): - membrane separation, spheroplasts, periplasmic proteins (reply: 1)