marker proteins for plasm membrane in c2c12 myoblasts - (Jan/23/2008 )
Hi folks
I am going to do the subcellular fractionation to seperate proteins in cell membrane, cytosol and nuclei of c2c12 myoblasts. I am wondering if there is any markers for membrane fraction, like histones as a marker for nuclear fraction
Thank you very much!
njdxsky
-njdxsky-
QUOTE (njdxsky @ Jan 23 2008, 10:54 PM)
Hi folks
I am going to do the subcellular fractionation to seperate proteins in cell membrane, cytosol and nuclei of c2c12 myoblasts. I am wondering if there is any markers for membrane fraction, like histones as a marker for nuclear fraction
Thank you very much!
njdxsky
I am going to do the subcellular fractionation to seperate proteins in cell membrane, cytosol and nuclei of c2c12 myoblasts. I am wondering if there is any markers for membrane fraction, like histones as a marker for nuclear fraction
Thank you very much!
njdxsky
Functionally and by Northern blot it was shown that C2C12 cells express the BMP receptor IA (BMPR-IA) which is a single transmembranic serine/threonine-kinase receptor which form heterotetramers with a Type II TGF/BMP receptor.
There are antibodies which specifically recognise this receptor (e.g. abcam).
maybe this helps.
-Dukes-