Will Yeast2Hybrid work on membrane proteins - (Nov/07/2006 )

I'm trying to run a yeast 2 hybrid screen on a protein that we're almost positive is membrane-bound (7 trans-membrane domains). So far it hasn't worked, but do you think that it's because the protein isn't being able to fold properly in the absence of a membrane to adhere to?

Your hypothesis could be right. Here in the lab we use a protein construction that have a myristoilation motif that permits the protein be translocated to plasma membrane. This makes more likely the interaction with your 7-transmembrane domains protein. Good Luck!