Methods needed (E.coli): - membrane separation, spheroplasts, periplasmic proteins (Mar/05/2002 )

I seek for new and reliable methods for E.coli:
Membrane separation, spheroplasts, periplasmic proteins extraction/detection and similar.
(links, articles, protocols – anything)
Thanks
lumax

Hi, You can see Clontech manual or Invitrogen or Qiagen
michel