|
181. Western blot using blood plasma - (reply: 5)
182. PelB Leader Sequence Cleavage - (reply: 1)
183. Probing protein - (reply: 1)
184. production of phosphorylated proteins in E.coli host system - (reply: 1)
185. What is the reason of doing proteomics work? - (reply: 2)
186. Protein quantification - (reply: 4)
187. Protein extraction for 2D electrophoresis - (reply: 2)
188. Protein extraction buffer preparation (please check for accuracy) - (reply: 6)
189. Test for protein in lysate - (reply: 4)
190. co-IP stained with coomassie blue - (reply: 2)
191. Why different elution buffers for Protein G and Protein A chromatography? - (reply: 1)
192. How many PBMCs should I seed so that I can get enough protein? - (reply: 1)
193. co-ip of endogenous and tagged proteins - (reply: 1)
194. Histone extraction from S.cerevisiae - (reply: 1)
195. coomassie blue stain - (reply: 1)
196. wise to do an enzyme kinetic assay with unpurified enzyme? - (reply: 11)
197. concentation calculations - (reply: 1)
198. Vesicular Monoamine Transporter2 (VMAT2) - (reply: 8)
199. Protein sequence conservation - (reply: 2)
200. MALDI MS resutls to identify proteins - (reply: 2)
201. Dye with thiol group - (reply: 1)
202. CoIP - (reply: 2)
203. GST pull down assay: GST binds to the prey protein. Negative control shows bindi - (reply: 4)
204. Problem in acetone precipitation of plant proteins. - (reply: 1)
205. Biotinylation - (reply: 1)
206. His Tagged Recombinant Protein not Recognized in Western Blot - (reply: 1)
207. Protein folding in different species - (reply: 1)
208. Immunoprecipitation- Incubation time with beads - (reply: 1)
209. desalting resin - (reply: 1)
210. immunoprecipitation concept - (reply: 4)
182. PelB Leader Sequence Cleavage - (reply: 1)
183. Probing protein - (reply: 1)
184. production of phosphorylated proteins in E.coli host system - (reply: 1)
185. What is the reason of doing proteomics work? - (reply: 2)
186. Protein quantification - (reply: 4)
187. Protein extraction for 2D electrophoresis - (reply: 2)
188. Protein extraction buffer preparation (please check for accuracy) - (reply: 6)
189. Test for protein in lysate - (reply: 4)
190. co-IP stained with coomassie blue - (reply: 2)
191. Why different elution buffers for Protein G and Protein A chromatography? - (reply: 1)
192. How many PBMCs should I seed so that I can get enough protein? - (reply: 1)
193. co-ip of endogenous and tagged proteins - (reply: 1)
194. Histone extraction from S.cerevisiae - (reply: 1)
195. coomassie blue stain - (reply: 1)
196. wise to do an enzyme kinetic assay with unpurified enzyme? - (reply: 11)
197. concentation calculations - (reply: 1)
198. Vesicular Monoamine Transporter2 (VMAT2) - (reply: 8)
199. Protein sequence conservation - (reply: 2)
200. MALDI MS resutls to identify proteins - (reply: 2)
201. Dye with thiol group - (reply: 1)
202. CoIP - (reply: 2)
203. GST pull down assay: GST binds to the prey protein. Negative control shows bindi - (reply: 4)
204. Problem in acetone precipitation of plant proteins. - (reply: 1)
205. Biotinylation - (reply: 1)
206. His Tagged Recombinant Protein not Recognized in Western Blot - (reply: 1)
207. Protein folding in different species - (reply: 1)
208. Immunoprecipitation- Incubation time with beads - (reply: 1)
209. desalting resin - (reply: 1)
210. immunoprecipitation concept - (reply: 4)